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Journal of Plant Nutrition and Fertilizers
2009,15
(3)
701-706
双阻NH_4~+选择性微电极测定水稻叶片细胞中NH_4~+的活度
作 者：
尹晓明;范晓荣;沈其荣
单 位：
江苏省固体有机废弃物资源化利用高技术研究重点实验室;南京农业大学资源与环境科学学院
关键词：
双阻NH_4~+选择性微电极;水稻叶片;细胞;NH_4~+
摘 要：
本文介绍了双阻NH_4~+选择性微电极的制作方法、工作原理及操作方法。微电极电位响应值与溶液中NH_4~+的活度呈对数曲线的关系,NH_4~+选择性微电极与其他类型的电极(如H~+、NO_3~-)最大区别是K~+的干扰,在含有72 mmol/L K~+的标定溶液中,电极标定曲线的斜率为48～58 mV,对NH_4~+的检出限小于10~(-4)mol/L,说明电极对NH_4~+有较高的选择性,受K~+的影响较小,可以用来测定。用以测定2.5 mmol/L NH_4~+培养2周的水稻叶片,结果表明,叶片细胞中NH_4~+活度分布在高低不同的两个区间内,分别代表了细胞质和液泡中的测定,水稻叶片细胞质和液泡NH_4~+的活度分别为2.58～9.30 mmol/L和11.36～25.20 mmol/L。水稻叶片组织中的NH_4~+主要来自液泡,流动分析仪测定的水稻叶片组织的NH_4~+浓度为11.12 mmol/L。NH_4~+选择性微电极为研究水稻对NH_4~+的吸收利用提供了技术支撑。
译 名：
Measurement of NH_4~+ activity in leaf cells of rice using double-barreled NH_4~+ selective microelectrodes
作 者：
YIN Xiao-ming,FAN Xiao-rong,SHEN Qi-rong~* (College of Recourses and Environmental Science/Jiangsu Provincial Key Laboratory of Organic Solid Waste Utilization, Nanjing Agricultural University,Nanjing 210095,China)
关键词：
double-barreled NH_4~+-selective microelectrodes;;rice leaf;;cell;;NH_4~+
摘 要：
This paper reported the manufacture method,working principle and operating method of double-barreled NH_4~+ selective microelectrode.An apparent difference of NH_4~+ selective microelectrode from other microelectrodes(such as H~+ and NO_3~- microelectrode) is the interference of K~+.These microelectrodes showed a typical log linear response to NH_4~+ from 0.01-100 mmol/L in the presence of 72 mmol/L K~+,with a slope of 48～58 mV.The detect limit was below 10~(-3) mmol/L.The results showed that the microelectrode was more sensitive and selective to NH_4~+ and could be used on the NH_4~+ measurement.Intracellular measurement of NH_4~+ activity in leaf cells of rice using the double-barreled NH_4~+ selective microelectrode showed that NH_4~+ activity fell into two main populations,one was in the cytoplasm with the NH_4~+ concentrations of 2.58-9.37 mmol/L and the other one was in the vacuole with the NH_4~+ concentrations of 11.36- 25.20 mmol/L.Tissue NH_4~+ of rice leaf mainly comes from that of the vacuole.Tissue NH_4~+ concentrations of rice leaf using a continuous-flow auto analyzer were 11.12 mmol/L.NH_4~+ selective microelectrode offered technical support for the study of the assimilation and utilization of NH_4~+ in rice.
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