关键词：
二乔木兰;花粉;萌发率;硼酸;钙离子;保存方法
摘 要：
通过使用不同的培养基,采用液体培养法研究了蔗糖、硼酸和钙离子对二乔木兰花粉萌发的影响。将所用花粉分为两组,组1于8:00采集,组2于16:00采集,结果表明:一定浓度的蔗糖、硼酸和钙离子对花粉的萌发有较大的促进作用,但超过一定浓度时则起抑制作用,二乔木兰花粉萌发的最适培养基为30 g·L~-1蔗糖+100mg·L~-1硼酸+500 mg·L~-1Ca~2+,在该培养基内,组1花粉的萌发率为46．21%±1．86%,花粉管长度为1156．25Um;组2花粉的萌发率仅为14．22%±1．86%,花粉管长度为1022um。此外,在其离体萌发过程中,二乔木兰花粉存在花粉管双萌发的现象。用不同的花粉保存方法保存二乔木兰花粉后,发现干燥处理后于-18℃下保存的花粉活性最高,30d后仍有6．92%±0．75%的萌发率,而于常温25℃下保存的花粉10d后就基本失活,说明低温干燥保存是保存二乔木兰花粉活性的有效途径。
译 名：
Study on Germination Property and Storage Conditions of Magnolia soulangeana Pollen
作 者：
HUANG Li-li SUN Peng SUN Bin LU Hong-fei (Zhejiang Normal University,Jinhua 321004,China)
关键词：
Magnolia soulangeana;;pollen;;germination rate;;boric acid;;calcium ion;;storage
摘 要：
Study on effect of sucrose,boric acid and calcium ion on germination of Magnolia soulangeana pollen by 19 kinds of culture medium.Pollen grains were collected at 8:00 as group 1 and collected at 16:00 as group 2.The result showed that sucrose,boric acid and calcium ion could promote pollen germination and tube growth at certain concentration.The optimal medium was 30g·L~-1 sucrose+100mg·L~-1 H_3BO_3+500mg.L~-1CaC1_2·2H_2O with 46.21% +1.86% and 1 156.25um pollen germination rate and tube length of group 1, with only 14.22%±1.86% and 1 022 um of group 2.Two pollen tubers from pollen were observed during in vitro germination.Study on storage conditions of M. soulangeana pollen resulted that the pollen stored at 18℃after drying could have survival rate of 6.92%±0．75% after 30 days.But that stored at 25℃died 10 days later.It demonstrated that pollen grains of the plant should be stored at conditions with low temperature after drying.
