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当前位置: 首页 > 文章 > 披碱草×野大麦杂种F_1幼穗培养再生体系的建立 中国草地学报 2016,38 (3) 14-18
Position: Home > Articles > Establishment of Plant Regeneration System by Culture of Yong Spike of Elymus dahuricus×Hordeum brevisubulatum Hybrid F_1 Chinese Journal of Grassland 2016,38 (3) 14-18

披碱草×野大麦杂种F_1幼穗培养再生体系的建立

作  者：
云玲格;李造哲;马青枝;谢菲;李月强
单  位：
内蒙古包头市达茂旗林业局;内蒙古农业大学生态环境学院;内蒙古农业大学生命科学学院;内蒙古阿拉善盟林木种苗站
关键词：
披碱草;野大麦;属间杂种F1;幼穗;组织培养;再生体系
摘  要：
以披碱草×野大麦杂种F_1幼穗为外植体,诱导出胚性愈伤组织,建立了植株再生体系,对影响愈伤组织诱导、分化、生根的基本培养基、激素种类及质量浓度等进行筛选。结果表明:愈伤组织诱导以MS为基本培养基+3mg/L 2,4-D较好;外植体在MS+3mg/L 2,4-D+30g/L蔗糖+7g/L琼脂的诱导培养基上经过20d培养,小穗愈伤组织诱导率达28.3%;愈伤组织的分化培养基为MS+1mg/L 6-BA+1mg/L NAA+30g/L蔗糖+7g/L琼脂,愈伤组织分化率达78%;生根培养基以1/2MS+0.5mg/L IBA+0.5mg/L NAA+蔗糖+7g/L琼脂为最佳,生根率可达86.7%,移栽后成活率可达100%。
译  名：
Establishment of Plant Regeneration System by Culture of Yong Spike of Elymus dahuricus×Hordeum brevisubulatum Hybrid F_1
作  者：
YUN Ling-ge;LI Zao-zhe;MA Qing-zhi;Xie Fei;LI Yue-qiang;College of Ecology and Environmental Science,Inner Mongolia Agricultural University;College of Life Science,Inner Mongolia Agricultural University;Forest Tree Seedlings Standing of Alashan League;Forestry Bureau of Damao Banner;
关键词：
Elymus dahuricus;;Hordeum brevisubulatum;;Inter genera hybrids F1;;Young spike;;Tissue culture;;Regeneration system
摘  要：
Yong spikes of Elymus dahuricus× Hordeum brevisubulatum F_1 were used as explants,embryonic callus was induced and plant regeneration system was successfully established,the basic culture mediums,hormones and concentrations for callus induction,differentiation and rooting were screened.The results showed that the better callus induction medium was MS +3mg/L 2,4-D,the ratio of callus induction was 28.3% after cultured in the induction medium of MS+3mg/L 2,4-D+30g/L surose+7g/L agar for 20days;the callus differentiation medium was MS+1mg/L 6-BA+1mg/L NAA+30g/L sucrose+7g/L agar,the ratio of callus differentiation was 78%;the optimal rooting medium was MS+0.5mg/L IBA+0.5mg/L NAA+sucrose+7g/L agar,rooting ratio was 86.7%;the survival rate after transplanting was 100%.

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