中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 玉米-大豆间作对玉米根际氨氧化微生物的影响 江苏农业学报 2017 (6) 1278-1287
Position: Home > Articles > Effects of maize-soybean intercropping on the ammonia oxidizing microbes in the rhizosphere of maize Jiangsu Journal of Agricultural Sciences 2017 (6) 1278-1287

玉米-大豆间作对玉米根际氨氧化微生物的影响

作  者：
刘丽;杨静;李成云
单  位：
西南林业大学生物多样性保护与利用学院云南省森林灾害预警与控制重点实验室;云南生物资源保护与利用国家重点实验室
关键词：
间作;玉米根际;氨氧化微生物;丰度;多样性
摘  要：
以氨单加氧酶基因(amo A)为分子标记,采用real time PCR和克隆文库的方法,研究玉米-大豆间作对玉米根际氨氧化古菌(AOA)和氨氧化细菌(AOB)的丰度、群落多样性及系统发育的影响。结果表明,间作玉米根际总细菌和AOA的数量均显著高于单作玉米根际。概念种或操作分类单元(OTUs)分析结果表明,间作玉米根际AOA和AOB的多样性均高于单作玉米根际。间作玉米根际AOA共获得23个OTU,AOB共获得16个OTU,单作玉米根际AOA和AOB分别获得13个OTU和20个OTU。系统发育分析结果表明,间作抑制了玉米根际AOB的Nitrosomonadaceae类群生长,促进了Nitrosospira cluster 3a类群和1个新类群的生长。AOA则在间作的玉米根际下分散到不同分支中,并在一定程度上促进了cluster water类群和cluster 1类群的生长,抑制了cluster 6类群的生长。
译  名：
Effects of maize-soybean intercropping on the ammonia oxidizing microbes in the rhizosphere of maize
作  者：
LIU Li;YANG Jing;LI Cheng-yun;State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan Agricultural University;College of Biodiversity Conservation and Utilization,Southwest Forestry University,Key Laboratory of Forest Disater Warning and Control of Yunnan Province;
关键词：
intercropping;;maize rhizosphere;;ammonia-oxidation microorganism;;abundance;;diversity
摘  要：
The abundance and composition of maize rhizosphere ammonia-oxidizing bacteria(AOB) and ammonia-oxidizing archaea(AOA) were investigated by using quantitative real-time polymerase chain reaction,cloning and sequencing approaches based on amo A genes. Intercropping maize rhizosphere had a higher copy numbers of 16 S rRNA and AOA amo A genes than the monoculture maize rhizosphere. Operational taxonomic unit(OTU) analysis results showed that the diversity of AOA and AOB was improved by intercropping. The number of OTU was 23 of AOA and 16 of AOB in intercropping maize rhizosphere. In monoculture maize rhizosphere,the number of OTU was 13 of AOA and 20 of AOB. Phylogenetic analyses of the amo A gene fragments showed that the intercropping inhibited the Nitrosomonadaceae group of AOB and cluster 6 of AOA,but promoted the Nitrosospira cluster 3 a and a new lineage of AOB and the cluster water and cluster 1 of AOA.

相似文章

导出引用
计量
文章访问数: 4
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46