作 者：
Dengfeng Zou;Xie AiZe;Qiu Yu-ting;Kai Yang;Yi Chen;Yan Zhan
单 位：
First Affiliated Hospital of Guangxi Medical University,Nanning,Guangxi 530022,Chin;First Affiliated Hospital of Guangxi Medical University,Nanning,Guangxi 530022,China
关键词：
emodin;polygonum perfoliatum l.;the content;999;accurate;4
摘 要：
[Objective] To extract and determine the content of emodin in Polygonum perfoliatum L.[Method] The content of emodin was determined by reverse phase HPLC.A column of Kromasil C18 was used and a mixture of methanol-water-glacial acetic acid(87∶13∶215) was used as the mobile phase at a flow rate of 110 ml/min.The UV detection wavelength was 254 nm and the column temperature was room temperature.[Result] The emodin showed a good linear relationship within the range of 1-40 μg/ml(r=0.999 9) ;the linear regression equation was Y=112.89X-8.912(r=0.999 9) .The optimum extraction technology was 80% ethanol concentration,35 ml ethanol dosage and 2 h extraction time.The average recovery rate was 99.16%(RSD=1.49%) .[Conclusion] The method was simple,rapid and accurate.It could be used for the extraction and content determination of emodin from Polygonum perfoliatum L.
