作 者：
Yadong Wei;Ming Dai;Wang Shuangxia;Guanghui Wang;Tengxiao Zhang;Lin H
单 位：
College of Biotechnology and Food Engineering,Sui Hua University,Heilongjiang Province,Suihua 152061,Chin;College of Biotechnology and Food Engineering,Sui Hua University,Heilongjiang Province,Suihua 152061,China
关键词：
active substances;lysing;algae;a1;crude extract;strai
摘 要：
Algae-lysing bacteria A1 has good control effect on the moss through its extracellular secretion.In order to further study its control mechanism,the single-factor tests of temperature and pH value,the activated carbon adsorption test,the organic solvent extraction test and the crude extract of active substance test were carried out to explore the characteristics of active substances.The results showed that the algae-lysing active ingredient of A1 strain had strong thermal stability,which still had good algae-lysing effect after being treated at 121 ℃;when the pH values of fermentation liquid were adjusted to 2.0 and 4.0 respectively,the active substances lost their activities,but their control ability against moss would be enhanced under the alkaline conditions;the active substances could not be adsorbed by activated carbon;the algae-lysing active substances showed strong hydrophilic ability when they were extracted by ethyl acetate,petroleum ether and chloroform.Therefore,it could be speculated that the active substances belonged to carbohydrates.The crude extract results of algae-lysing active substances showed that the active substances secreted by A1 strain were composed by a variety of algae-lysing active substances.
