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当前位置: 首页 > 文章 > 砂糖橘精油化学组成及对指状青霉的抑菌活性 食品科学 2013,34 (7) 125-128
Position: Home > Articles > Chemical Composition and Antifungal Activity against Penicillium digitatum of Essential Oil from C. reticulate Blanco FOOD SCIENCE 2013,34 (7) 125-128

砂糖橘精油化学组成及对指状青霉的抑菌活性

作  者：
杨彦松
单  位：
湘潭大学化工学院
关键词：
精油;GC-MS;指状青霉;抑菌活性;C.reticulate Blanco
摘  要：
采用水蒸气蒸馏法提取砂糖橘(Citrus reticulate Blanco)果皮精油,并用GC-MS对其成分进行分析,共鉴定出17种成分,主要包括柠檬烯(77.99%)、β-月桂烯(3.74%)、β-水芹烯(2.78%)、罗勒烯(2.64%)、β-芳樟醇(2.35%)、α-蒎烯(1.60%)、正辛醇(1.54%)、癸醛(1.24%)、4-松油醇(1.10%)和4-十一烯(1.04%)等.测定不同添加量(0.16、0.31、0.63、1.25、2.50、5.00、10.00μL/mL)砂糖橘精油对指状青霉孢子萌发和菌丝体生长的作用.结果表明:低添加量(<2.5μL/mL)砂糖橘精油对指状青霉孢子萌发无影响甚至有促进作用,对指状青霉菌丝体生长有轻微的抑制;而高添加量(10μL/mL)则显著抑制孢子萌发和菌丝体生长(P<0.05).本实验表明砂糖橘精油在防治指状青霉具有一定作用.
译  名：
Chemical Composition and Antifungal Activity against Penicillium digitatum of Essential Oil from C. reticulate Blanco
单  位：
College of Chemical Engineering,Xiangtan University,Xiangtan 411105,China
关键词：
essential oil%GC-SM%Penicillium digitatum%antifungal activity%C.reticulate Blanco
摘  要：
Essential oil of Shatangju(C.reticulate Blanco) was extracted from the fruit peel by hydro-distillation and analyzed by GC-MS.As a result,17 different components were identified.The major components were limonene(77.99%),followed by β-myrcene(3.74%),β-phellandrene(2.78%),ocimene(2.64%),β-linalool(2.35%),α-pinene(1.60%),1-octanol(1.54%),decanal(1.24%),4-terpineol(1.10%) and 4-undecene(1.04%).The antifungal activity of the essential oil at different concentrations(0.16,0.31,0.63,1.25,2.5,5.0 μL/mL and 10.0 μL/mL) against the spore germination and mycelia growth of P.digitatum was determined.Results showed that the oil at lower concentrations(< 2.5 μL/mL) had no obvious or even stimulatory effect on the spore germination of P.digitatum,but slightly inhibited the mycelia growth of P.digitatum.In contrast,the oil at a higher concentration(10 μL/mL) remarkably inhibited both the spore germination and mycelia growth of P.digitatum.This study indicates that Shatangju essential oil plays a positive role in controlling P.digitatum.

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