当前位置: 首页 > 文章 > 香蜂花的离体保存及试管苗抗氧化酶活性的变化 福建农林大学学报(自然科学版) 2014,43 (3) 41-46
Position: Home > Articles > In vitro conservation and the changes of antioxidase activity of Melissa officinalis plantlets Journal of Fujian Agriculture and Forestry University(Natural Science Edition) 2014,43 (3) 41-46

香蜂花的离体保存及试管苗抗氧化酶活性的变化

作  者:
姚凤琴;赖钟雄
单  位:
福建农林大学园艺植物生物工程研究所;三明市农业科学研究院
关键词:
香蜂花;离体保存;抗氧化酶活性
摘  要:
以香蜂花试管苗为材料,研究不同浓度ABA和不同因素(白糖、琼脂、甘露醇、多效唑)对香蜂花试管苗离体保存的影响,并且测定试管苗在最佳培养基保存过程中抗氧化酶活性的变化.结果表明:适宜香蜂花试管苗离体保存的最佳培养基为MS+20 g·L-1白糖+9 g·L-1琼脂+10 g·L-1甘露醇+7 mg·L-1多效唑;同时发现最佳培养基与对照MS培养基中试管苗的抗氧化酶(SOD、CAT及POD)活性的变化趋势都是先上升后下降,但最佳培养基上的试管苗的酶活性要高于对照并且延迟了酶活性最高峰出现的时间.
译  名:
In vitro conservation and the changes of antioxidase activity of Melissa officinalis plantlets
作  者:
YAO Feng-qin;LAI Zhong-xiong;Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University;Sanming Institute of Agricultural Sciences;
关键词:
Melissa officinalis;;in vitro conservation;;antioxidase activity
摘  要:
The effects of in vitro conservation on Melissa officinalis plantlets under different concentrations of ABA and different factors( sugar,agarophyte,mannitol,paclobutrazol),and the changes in activities of antioxidase of the plantlets from different media were examined.The results showed that the best medium for in vitro conservation of Melissa officinalis plantlets was MS medium with 20 g·L- 1sugar,9 g·L- 1agarophyte,10 g·L- 1mannitol and 7 mg·L- 1 paclobutrazol.Additionally,trends of these antioxidase( SOD,CAT,and POD) activities in vitro conservation of Melissa officinalis plantlets on both the best medium and the MS medium were accordant,rose at first and then declined.But the antioxidase activity of the plantlets kept on the best medium was higher than that on the MS medium and delayed the time of the peak of enzyme activity.

相似文章

计量
文章访问数: 13
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊