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当前位置: 首页 > 文章 > 细胞自噬对松材线虫繁殖存活的影响及线虫与松树互作早期自噬相关基因的表达 南京林业大学学报(自然科学版) 2018 (5) 59-64
Position: Home > Articles > The role of autophagy of pine wood nematode Bursaphelenchus xylophilus in the growth and development and infecting pine trees at early time Journal of Nanjing Forestry University(Natural Sciences Edition) 2018 (5) 59-64

细胞自噬对松材线虫繁殖存活的影响及线虫与松树互作早期自噬相关基因的表达

关键词：
松材线虫;细胞自噬;自噬诱导剂;雷帕霉素;外源诱导;基因表达
摘  要：
【目的】细胞自噬在真核生物细胞应对不良环境中具有重要作用。探讨细胞自噬对松材线虫取食繁殖的影响,测定松材线虫与松树互作过程中松材线虫Bx ATG1和Bx ATG8两个自噬相关基因的表达。【方法】在前期研究发现松材线虫存在细胞自噬现象,并克隆了松材线虫Bx ATG1和Bx ATG8两个自噬相关基因的基础上,采用外源自噬诱导剂探讨了细胞自噬对松材线虫取食繁殖的影响,测定了松材线虫与松树互作早期自噬相关基因的表达。【结果】经雷帕霉素处理后,松材线虫的自噬启动基因Bx ATG1和自噬标志性基因Bx ATG8表达量显著上调,且松材线虫的取食速率及繁殖量也显著增加;松材线虫在饥饿状态下的存活能力明显上升,脂肪含量的堆积也略有增加。松材线虫与松树互作早期(6、12、18、24 h)的qRT-PCR结果表明,松材线虫侵染松树12 h时,细胞自噬爆发达到最高峰。【结论】外源自噬诱导后促进了松材线虫细胞自噬的发生;细胞自噬在促进松材线虫生长发育中具有一定作用;在松材线虫侵染松树过程中,细胞自噬有助于线虫适应松树的早期抵抗反应。
译  名：
The role of autophagy of pine wood nematode Bursaphelenchus xylophilus in the growth and development and infecting pine trees at early time
作  者：
WU Fan;WU Xiaoqin;Co-Innovation Center for the Sustainable Forestry in Southern China,Jiangsu Key Laboratory for Prevention and Management of Invasive Species,College of Forestry,Nanjing Forestry University;
单  位：
WU Fan%WU Xiaoqin%Co-Innovation Center for the Sustainable Forestry in Southern China,Jiangsu Key Laboratory for Prevention and Management of Invasive Species,College of Forestry,Nanjing Forestry University
关键词：
Bursaphelenchus xylophilus;;autophagy;;autophagy inducer;;Rapamycin;;exogenous induction;;gene expression
摘  要：
【Objective】Autophagy plays an important role in Eukaryotic cells adapting adversities. Nevertheless,there're few researches into autophagy and its functions in pine wood nematode( PWN),which is the pathogeny of pine wilt disease. 【Method】Our laboratory previously found that there existed autophagy in PWNs,thus,we cloned genes Bx ATG1 and Bx ATG8 of them,as the genes were related to autophagy. Based on that,this study discussed the impact of autophagy on PWNs' growth and development,as well as its roles during the interaction between PWNs and pines. 【Result】After being treated with Rapamycin,which was the autophagy inducer,the expression of Bx ATG1 and Bx ATG8 rose significantly,which demonstrated that the exogenous autophagy induction had promoted the occurrence of autophagy. Besides,the exogenous autophagy inducer notably helped with the boost in PWNs' feeding rate and reproduction. What's more,after autophagy induction,PWNs' viability under starving condition was stimulated obviously,and the accumulation of fats also improved. These facts showed that autophagy played an important role in the growth and development of PWNs.qRT-PCR results of early interaction between PWNs and pines showed that,the occurrence of autophagy reached its climax after infecting the pine seedlings for 12 hours,which implied that,during the course of PWNs infecting the pines,autophagy was helpful for PWNs to go through the early stage of plant's resistance response.【Conclusion】Induction of autophagy promoted the occurrence of PWN's autophagy,and autophagy played an important role in promoting the growth and development of Bursaphelenchus xylophilus. In the process of pine wood nematode infestation,autophagy can help the nematode adapt to the early resistance of pine.

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