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Position: Home > Articles > Isolation and Identification of Protease-producing Srains from Luzhou-flavor Liquor Starter Anhui Agricultural Science Bulletin 2017,23 (21) 29-31

浓香型高温酒曲中蛋白酶产生菌的筛选与鉴定

作  者:
黄家驷;刘盾;陈玄阳;王珊珊;陈燕;程爽
单  位:
南阳理工学院生物与化学工程学院
关键词:
酒曲;蛋白酶;筛选;鉴定
摘  要:
该研究利用酪蛋白选择性培养基从浓香型高温酒曲中分离得到1株有明显水解圈的蛋白酶产生菌LS-1,经16SrDNA鉴定其为地衣芽孢杆菌;LS-1通过发酵培养提取出粗酶液,其酶活为180U/mL。
译  名:
Isolation and Identification of Protease-producing Srains from Luzhou-flavor Liquor Starter
作  者:
Huang Jiasi;School of Biological and Chemical Engineering,Nanyang Institute of Technology;
单  位:
School of Biological and Chemical Engineering,Nanyang Institute of Technology
关键词:
Liquor Starter;;Protease;;Isolation;;Identification
摘  要:
A protease-producing strain was isolated from Luzhou-flavor Liquor Starter with casein-selective medi-um,named LS-1. The 16 Sr DNA was amplified by PCR and the amplification products was subjected to sequencingand homology analysis. As a result,strain LS-1 was identified as Bacillus licheniformis. The protease activity in fer-mentation broth was 180 U/m L.

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