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当前位置: 首页 > 文章 > 藏绵羊ANGPTL4基因第3内含子、第6外显子多态性分析 江西农业大学学报 2017 (3) 443-449
Position: Home > Articles > Polymorphism of ANGPTL4 Gene in Tibet Sheep Acta Agriculturae Universitatis Jiangxiensis 2017 (3) 443-449

藏绵羊ANGPTL4基因第3内含子、第6外显子多态性分析

作  者：
李文浩;刘秀;李少斌;王继卿;罗玉柱
单  位：
甘肃农业大学动物科学技术学院/甘肃省草食动物生物技术重点实验室
关键词：
高寒牧区;藏绵羊;ANGPTL4;多态性
摘  要：
为分析高寒牧区藏绵羊ANGPTL4基因多态性,应用PCR-SSCP与测序结合的方法,对391只藏绵羊ANGPTL4基因第3内含子、第6外显子进行多态性检测。经扩增,分别获得大小为280 bp和289 bp的2个片段。ANGPTL4基因第3内含子发现AA、BB和AB 3种基因型,各基因型频率分别为0.648 8、0.313 0和0.038 2,在C.400+90处发生G/T的突变,多态信息含量为0.265 6,呈中度多态。ANGPTL4基因第6外显子发现AA和AB2种基因型,基因型频率分别为0.961 8和0.038 2,在C.837处发生C/T的突变,为错义突变,并导致P.279处苏氨酸变为丝氨酸,多态信息含量为0.036 9,为低度多态。对藏绵羊ANGPTL4基因的多态性进行分析,发现的2处突变位点可作为藏绵羊品种改良以及种质资源保护的潜在分子标记。
译  名：
Polymorphism of ANGPTL4 Gene in Tibet Sheep
作  者：
LI Wen-hao;LIU Xiu;LI Shao-bin;WANG Ji-qin;LUO Yu-zhu;Faculty of Animal Science and Technology,Gansu Agricultural University/Gansu Key Laboratory of Herbivorous Animal Biotechnology;
关键词：
alpine pastoral areas;;Tibetan sheep;;ANGPTL4;;polymorphism
摘  要：
In order to study the gene polymorphism of Tibetan sheep ANGPTL4 gene,391 copies of Tibetan sheep blood samples were and collected their genomic DNA were extracted.The polymorphism of intron-3 and exon-6 of the ANGPTL4 was tested by means of PCR-SSCP combined with sequencing.By amplification two fragments in size of 280 bp and 289 bp were obtained. Primer amplification products by the modified polypropylene 3 belt types,namely AA,BB and AB,were found from the intron-3 of ANGPTL4 gene,and their genotype frequencies were 0.648 8,0. 313 0 and 0. 038 2,respectively. The polymorphism information content was0.265 6,belonging to moderate polymorphism. Individual test showed that the primer amplification fragment of P1 was 1 base mutation,G/T mutation was found in c.400 G+90; In primer amplification of P2 product by the modified polypropylene amide gel electrophoresis,two types appeared,namely AA and AB,their genotype frequencies were 0.961 8 and 0.038 2,respectively.The polymorphism information content was 0.036 9 belonging to low polymorphism.Individual test showed that the primer amplification fragment of P2 was 1 base mutation,C/T mutations occurred,which in C.837/P.279 led in lead to the corresponding amino acid change.The amplification loci found in this study can be used as potential molecular markers for quality improvement and germ plasm protection of Tibetan sheep.

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