中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 玉米叶片受新月弯孢菌侵染后的细胞病理学变化 植物病理学报 2004,34 (1) 21-26
Position: Home > Articles > Cytopathological changes of maize leaf tissue infected by Curvularia lunata (Wakker) Boed Acta Phytopathologica Sinica 2004,34 (1) 21-26

玉米叶片受新月弯孢菌侵染后的细胞病理学变化

作  者：
黄丽丽;王兰;康振生;赵杰;张管曲
单  位：
西北农林科技大学植保学院
关键词：
玉米;弯孢菌叶斑病;超微结构;细胞化学;细胞壁降解酶
摘  要：
本文利用透射电子显微镜技术与细胞化学技术研究了玉米叶片受弯孢菌侵染后的超微结构和细胞壁的组成成份变化。透射电镜观察发现,病菌侵入后,菌丝先在寄主细胞间扩展,随着寄主细胞病变、坏死,菌丝可进入寄主细胞形成胞内菌丝。随病菌侵入和在寄主体内扩展,寄主细胞先后发生了一系列的超微结构变化,叶绿体、液泡等细胞器解体,出现质壁分离现象,并最终解体、坏死、变形。细胞化学标记定位发现,受侵寄主细胞壁中纤维素、木聚糖和果胶质的标记密度明显低于未接种的健康组织,表明细胞壁降解酶(如纤维素酶、木聚糖酶和果胶酶)的产生与病菌侵染和致病过程密切相关。
译  名：
Cytopathological changes of maize leaf tissue infected by Curvularia lunata (Wakker) Boed
作  者：
HUANG Li-li, WANG Lan, KANG Zhen-sheng , ZHAO Jie, ZHANG Guan-qu (College of Plant Protection, Northwest Sci-Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100, China; 2Plant Sci-Tech College, Tarim Reclamation University of Agriculture, Alar, Xinjiang 843300, China)
关键词：
maize; Curvularia lunata; ultrastructure; cytochemistry; cell wall-degrading enzymes
摘  要：
The transmission electron microscope and cytochemical technique were employed to examine the alteration of ultrastructure and cell wall components of maize leaf tissue, uninfected and infected by Curvularia lunata (Wakker) Boed. After invading into the maize leaf tissue, the pathogen spread inter-cellularly and then formed intracellular hyphae in the necrotic host cells. As the extension of the hy-phae, a series of cytopathological changes occurred in the host tissue, including plasmolysis, necrosis of protoplasm, degeneration of organelles such as chloroplasts and vacuoles, and collapse of host cells. The cytochemical labeling of main cell wall components in C. lunata-infected leaf tissue showed that the labeling densities for cellulose, xylan, pectin were significantly reduced as compared with that in unino-culated healthy tissue. These results indicate that cell wall-degrading enzymes such as cellulases, xy-lanases, pectinases secreted by C. lunata are closely related to the penetration and extension of pathogen.

相似文章

导出引用
计量
文章访问数: 4
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46