当前位置: 首页 > 文章 > 油菜HY5基因的电子克隆及生物信息学分析 北方园艺 2013 (23) 116-118
Position: Home > Articles > In silico Cloning and Bioinformatics Analysis on HY5 Gene of Brassica napus Northern Horticulture 2013 (23) 116-118

油菜HY5基因的电子克隆及生物信息学分析

作  者:
郭继平
单  位:
衡水学院生命科学系
关键词:
HY5;油菜;光受体;电子克隆
摘  要:
利用已知的芜菁HY5编码基因序列为探针,采用电子克隆的方法获得了油菜HY5基因cDNA的序列。采用生物信息学工具对其编码蛋白序列进行了研究,并将其编码蛋白同芜菁和拟南芥的HY5蛋白进行了同源性比较,构建了其系统发育树。结果表明:油菜HY5基因编码164个氨基酸,其分子量为18 037.8Da,是亲水蛋白,同芜菁HY5具有高度的同源性,高于和拟南芥的同源性;该试验初步获得了油菜HY5基因的编码序列,为进一步研究其功能打下基础。
译  名:
In silico Cloning and Bioinformatics Analysis on HY5 Gene of Brassica napus
作  者:
GUO Ji-ping;Department of Life Sciences,Hengshui College;College of Food Science and Engineering,Harbin Institute of Technology;
关键词:
HY5;;Brassica napus;;photoreceptor;;in silico cloning
摘  要:
With HY5gene in Brassica rapaas probe,the cDNA of HY5gene of Brassica napus was in silico cloned.Using bioinformaticstool,amino acid sequence coded by HY5of Brassica napus was predicted and homology compared with Arabidopsis thaliana and Brassica rapa.Its phylogenetic tree was constructed.The results showed that HY5of Brassica napus coded a protein of 164amino acids.The calculated molecular weight of it was 18 037.8Da.It was a hydrophilic protein.The homology of HY5protein between Brassica napus and Brassica rapa was higher than that between Brassica napus and Arabidopsis thaliana.The HY5gene coding sequence of Brassica napus was cloned preliminary,and laid the foundation for further study its function.

相似文章

计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊