单 位：
辽宁省农业科学院玉米研究所;沈阳农业大学;沈阳农业大学生物科学技术学院辽宁省农业科学院玉米研究所;沈阳农业大学生物科学技术学院辽宁省农业科学院植物保护研究所
关键词：
Ac/Ds转座子;转座特点;转座机制;转座子标签
摘 要：
Ac/Ds转座子作为研究较为深入的植物转座子,已被广泛应用于植物功能基因组研究。利用Ac/Ds标签系统构建插入突变体库是研究功能基因组学的有效途径,也是进行基因克隆及功能分析的有利工具。结合课题组利用Ac/Ds转座子标签分离基因并进行基因功能验证方面的研究工作,综述了Ac/Ds转座子在植物基因组中的插入特点、转座机制以及转座子标签法的应用与发展前景,为更有效的利用Ac/Ds转座子提供参考依据。
译 名：
Research Progress about Ac/Ds Transposon in Plant
作 者：
FAN Ming-xia1,JIANG Min2,WANG Guo-hong2,WANG Dong-xu1,ZHOU Ting1, ZHANG Chun-yu1,LIN Feng1 (1.College of Biolosical Science and Technology,Shenyang Agricultural University,Shenyang 110161,China;2.Maize Institute,Liaoning Academy of Agricultural Sciences Shenyang 110161,China)
关键词：
Ac/Ds transpeson;character;transpodable mechanism;transpeson tagging
摘 要：
Activator/dissociation(Ac/Ds) transposons as a more in-depth study of plant transposons have been widely used in plant functional genome research.Ac/Ds tagging system is an important and effective way to construct insertion library for functional genome studies.It is also a powerful tool for gene cloning and functional analysis.Our research group also used Ac/Ds transposon tagging to isolate genes and analyse gene function.This article reviews the characters of Ac/Ds transposons,the transposable mechanism of Ac/Ds transposons,transposon tagging,as well as the application and developmental prospects of Ac/Ds transposon system.It also provides references for the application of Ac/Ds transposon system.
