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Position: Home > Articles > A Review of Recent Advances in the Application of DNA Barcoding in Identification of Animal Species in Highly Processed Animal-Derived Products MEAT RESEARCH 2017,31 (1) 48-54

DNA条形码技术在深加工动物制品源性成分鉴定中的应用研究进展

作  者：
凌胜男;吴亚君;韩建勋;乔旭光;陈颖
单  位：
中国检验检疫科学研究院农产品安全研究中心;山东农业大学食品科学与工程学院
关键词：
DNA条形码;深加工;动物制品;动物源性成分
摘  要：
DNA条形码被认为是一种强大、非定向、准确特异、低成本且适用广泛的方法,是分子生物学领域研究物种鉴定的热点技术。其基于DNA分子水平和通用引物扩增的鉴定方式规避了传统鉴别方法的不足,又超脱于许多分子检测方法只能定向检测的局限,现已被广泛应用于生态环境、医疗卫生、食品安全等各个领域。本文介绍了DNA条形码的基本原理、发展进程和研究现状,总结了其在深加工动物制品中的应用情况,包括在动物源性中药材、海产品、肉及肉制品、动物源性保健品以及皮革制品、动物标本等源性成分鉴别方面的应用,最后对DNA条形码技术鉴定物种的优势和不足以及发展趋势作了总结和展望。
译  名：
A Review of Recent Advances in the Application of DNA Barcoding in Identification of Animal Species in Highly Processed Animal-Derived Products
作  者：
LING Shengnan;WU Yajun;HAN Jianxun;QIAO Xuguang;CHEN Ying;Agro-product Safety Research Center, Chinese Academy of Inspection and Quarantine;College of Food Science and Engineering, Shandong Agricultural University;
关键词：
DNA barcoding;;deep processing;;animal-derived products;;animal-derived ingredients
摘  要：
DNA barcoding is considered to be a powerful, non-directional, specific, accurate and economical method that can be applied widely. As a technique of great interest for researchers to identify special origin in the field of molecular biology, DNA barcoding amplifies specific DNA sequences by using universal primers, which helps to overcome the shortcomings of the traditional detection methods and transcend the limitation of many molecular methods to directional detection. DNA barcoding nowadays has been widely applied in the fields of environmental and ecological protection, medicine and healthcare, and food safety. This paper outlines the basic principle, developmental process and current studies of DNA barcoding and summarizes its application in the detection of animal species in highly processed animal-derived products like animal-derived traditional Chinese medicinal materials, marine animal products, meat and meat products, animalderived health products, leather products, and animal specimens. Finally we conclude this review with the advantages and disadvantages of DNA barcoding as well as some perspectives on the future development trend of DNA barcoding.

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