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当前位置: 首页 > 文章 > 气相色谱质谱法测定孜然挥发油中枯茗醛含量的方法研究 食品科学 2009,30 (6) 208-212
Position: Home > Articles > GC-MS Determination of Cuminal Content in Volatile Oil of Cuminum cyminum L. FOOD SCIENCE 2009,30 (6) 208-212

气相色谱质谱法测定孜然挥发油中枯茗醛含量的方法研究

作  者：
杨文菊;杨涛;刘龙;于波;田树革;敬思群
单  位：
新疆大学生命科学与技术学院;新疆医科大学药学院;新疆产品质量监督检验研究院
关键词：
孜然;挥发油;GC-MS;枯茗醛
摘  要：
本研究利用水蒸气蒸馏法提取孜然挥发油中枯茗醛,并采用GC-MS法进行含量测定。采用岛津DB-5柱(30m×0.32mm,0.25μm),进样口温度220℃,色谱升温条件为起始温度40℃,升温速率8℃/min,终点温度220℃,氦气流量1.2ml/min,分流比100:1,进样量0.5μl。结果表明枯茗醛浓度在7.75～69.75mg/ml呈良好线性关系,线性方程Y=3.34×108X-3.373×106(r=0.9989),平均回收率96.5%,重复性RSD为1.8%(n=3)。此法简便、准确且重复性好,可为孜然质量标准的制定提供科学的方法依据。
译  名：
GC-MS Determination of Cuminal Content in Volatile Oil of Cuminum cyminum L.
作  者：
YANG Wen-ju1, YANG Tao1, LIU Long2,YU Bo2,TIAN Shu-ge2,JING Si-qun3 (1. Xinjiang College of Quality and Technical Supervision, Urumqi 830004, China ;2. College of Pharmacy, Xinjiang Medical University, Urumqi 830054, China ;3. College of Life Science and Technology, Xinjiang University, Urumqi 830046, China)
关键词：
Cuminum cyminum L. ;essential oil;GC-MS;cuminal
摘  要：
In this study, the volatile oil of Cuminum cyminum L. was extracted by steam distillation method and the cuminal content in the volatile oil was analyzed using GC-MS. The Shimazu Column DB-5 (30 m ×0.32 mm, 0.25 μm) was used. The injection temperature was 220 ℃, and the initial temperature of 40 ℃ was increased to 220 ℃ at the rising rate of 8 ℃/min. The helium flow rate, split ratio and injection volume were 1.2 ml/min, 100:1 and 0.5 μl, respectively. The results showed that there is good linear relationship between integral value (Y) of peak area and cuminal content (X) in the range of 7.75 to 69.75 mg/ ml, and the linear equation is Y = 3.34 × 108X - 3.373 × 106 (r =0.9989). The RSDs in precision, repeatability and stability experiments are 1.40%, 2.3% and 1.2%. The average spike recovery is 96.5% with the RSD of 1.8% (n=5). The method is simple and accurate with good reproducibility, therefore it can be used for quality control of Cuminum cyminum L..

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