作 者：
SU Xian-zhong;Yu Min;Ming Li;Quan-Xi Zheng;Liu ShouKu
单 位：
Department of Biological Engineering, School of Chemical Engineering, China University of Mining and Technology, Xuzhou, Jiangsu 221116, China;Department of Biological Engineering, School of Chemical Engineering, China University of Mining and Technology, Xuzhou, Jiangsu 221116, Chin
关键词：
radix paeoniae rubra;00;extracts;peoniflorin;antimicrobial;ethano
摘 要：
[Objective] The aim was to extract and determine the major antimicrobial active constituents from RADIX PAEONIAE RUBRA. [Method] RADIX PAEONIAE RUBRA was treated by ethanol ultrasonic extraction, HP20 colophony column chromatogram and other processes. And the in vitro antimicrobial tests on Klebsiella pneumoniae ATCC 49790, Acinetobacter baumannii 36 and Serratia marcescens ATCC 14041 were carried out by the two-fold dilution of extracts. FTIR spectra of the extracts were analyzed by Fourier transform infrared spectroscopy. [Result] 100% ethanol elution had the optimum inhibitory effects on the three strains, with the MICs of 32.00, 32.00 and 125.00 g/L, respectively. The FTIR spectrum of the extracts was very similar to that of peoniflorin. [Conclusion] Peoniflorin was the main active components of RADIX PAEONIAE RUBRA, and had significant antimicrobial effect.
