Position: Home > Articles > MSCs联合EPCs体外构建组织工程化血管的实验研究
Journal of Shihezi University(Natural Science)
2018
(2)
MSCs联合EPCs体外构建组织工程化血管的实验研究
作 者：
邢立行;李智伟;张中洲;王小义;姜慧娇;陈雪玲;吴向未
关键词：
聚羟基乙酸;间充质干细胞;内皮祖细胞;碱性成纤维细胞生长因子;组织工程化血管
摘 要：
目的为了探讨间充质干细胞(Mesenchymal stem cells,MSCs)联合内皮前体细胞(Endothelial progenitor cells,EPCs)在体外构建组织工程化血管的可行性以及碱性成纤维细胞生长因子(basic fibroblast growth factor,b FGF)的作用.方法首先分离、培养、扩增小鼠骨髓来源的MSCs和EPCs.剪裁、消毒、胶原包埋聚羟基乙酸(Polyglycolic acido,PGA)支架材料.按EPCs组、MSCs组、EPCs联合MSCs组、EPCs联合MSCs加b FGF培养组将各组细胞分别接种到处理过的PGA支架上制成细胞、PGA复合物,将以上制成的复合物在体外培养1周时分别用HE染色及倒置显微镜观察细胞与PGA的相容性,培养8周时做HE及免疫组织化学染色检测CD34、α-SMA蛋白水平,并对免疫组织化学染色评分后分析细胞、PGA复合物培养情况及各组之间的差异.结果 1周时,大量细胞贴附在PGA支架纤维上,细胞与PGA相容性良好;8周时各分组免疫组织化学α-SMA染色均为阴性;联合组免疫组织化学CD34染色阳性,并评分明显高于EPCs组和MSCs组,且差异有统计学意义(P<0.05);EPCs组染色评分明显高于MSCs组,且差异有统计学意义(P<0.05);b FGF组与联合组之间染色评分无明显差异(P>0.05).结论 MSCs联合EPCs可在体外构建组织工程化血管,两种细胞联合应用明显优于单种细胞应用,单独的b FGF应用没有明显的促进作用.
作 者：
Xing Lihang;Li Zhiwei;Zhang Zhongzhou;Wang Xiaoyi;Jiang Hui jiao;Chen Xueling;Wu Xiangwei;Department of General Surgery, the First Affiliated Hospital of Shihezi University School of Medicine;Department of Immunology, Shihezi University School of Medicine;
单 位：
Xing Lihang%Li Zhiwei%Zhang Zhongzhou%Wang Xiaoyi%Jiang Hui jiao%Chen Xueling%Wu Xiangwei%Department of General Surgery, the First Affiliated Hospital of Shihezi University School of Medicine%Department of Immunology, Shihezi University School of Medicine
关键词：
polyglycolic acid;;mesenchymal stem cells;;endothelial progenitor cells;;basic fibroblast growth factor;;tissue engineered blood vessel
摘 要：
Objective To study the feasibility of constructing tissue engineered blood vessels by co-culture of mesenchymal stem cells(MSCs) and endothelial progenitor cells(EPCs) in vitro and the role of basic fibroblast growth factor(b FGF). Methods Firstly, MSCs and EPCs were isolated, cultured and amplified from the bone marrow of mice. Polyglycolic acid(PGA) was tailored, sterilized and embedded in rat tail collagen. Experimental groups: EPCs group, MSCs group, EPCs combined with MSCs group, EPCs combined with MSCs and b FGF group. The cells of each group were inoculated on the PGA scaffold. The compatibility of cells with PGA was observed by HE staining and microscope after being cultured in vitro for 1 week. After 8 weeks, HE and immunohistochemical staining(CD34, alpha-SMA) are performed. R esults It was found that a large number of cells were attached to the PGA scaffold fiber and the cells were compatible with PGA after 1 week. α-SMA staining was negative in all groups after 8 weeks. The combined group was positive for CD34 staining, and the score was significantly higher than that of EPCs group or MSCs group. The difference was statistically significant(P<0.05). The CD34 staining score of EPCs group was significantly higher than that of MSCs group. The difference was statistically significant(P<0.05). There was no significant difference between b FGF group and the combined group(P>0.05). Conclusion MSCs combined with EPCs can construct tissue-engineered blood vessels in vitro. The combination of two kinds of cells is better than single cell application.However, the use of b FGF alone doesn't significantly promote the effect.
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