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Position: Home > Articles > Isolation and identification of high-quality lactic acid bacteria in forage corn. Animal Husbandry and Feed Science 2011,3 (1)

Isolation and identification of high-quality lactic acid bacteria in forage corn.

作  者：
Ping Yan;Yonghui Zhang;A. Mairemunisa;M. Abudukeyoumu;Yimiti Wusima
单  位：
Mairemunisa·Aimaier,Abudukeyoumu·Maimaiti,Wusiman·Yimiti College of Life Sciences and Technology,Xinjiang University,Urumqi 830046,China;Mairemunisa·Aimaier,Abudukeyoumu·Maimaiti,Wusiman·Yimiti College of Life Sciences and Technology,Xinjiang University,Urumqi 830046,Chin
关键词：
strains;forage corn;lactic acid bacterium;isolated;production ability;strong aci
摘  要：
[Objective] To screen suitable lactic acid bacterium strains from forage corn which can be used as silage additives.[Method] The lactic acid bacterium strains were isolated by inoculation on MRS solid medium containing calcium carbonate,and they were preliminarily identified through morphological,physiological and biochemical experiments.The acid production efficiency was determined.Twelve strains having strong acid-production ability were selected,and their salt tolerance and acid tolerance were detected.The sequences of their 16 S rDNA were also analyzed.[Result] A total of 44 lactic acid bacterium strains were isolated from the forage corn.As evidenced by the physiological and biochemical experiments,the twelve strains having strong acid-production ability belonged to Leuconostoc,Lactobacillus and Enterococcus,respectively,and they had strong salt tolerance and acid tolerance.According to the sequences of 16 S rDNA,A4,B9,B11,B12 and B14 were Lactobacillus plantarum;A1,A2,A7,A11 and B8 were Leuconostoc mesenteroides dextran subspecies;and A8 and A9 were Enterococcus hirae.[Conclusion] The lactic acid bacterium strains with strong acid-production ability isolated from forage corn can be developed into silage additives.

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