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当前位置: 首页 > 文章 > 太白贝母多糖分离及结构表征 西南大学学报(自然科学版) 2020 (6) 11-19
Position: Home > Articles > Separation and Structure Characterization of Polysaccharides in Fritillaria taipaiensis P.Y.Li Journal of Southwest University(Natural Science Edition) 2020 (6) 11-19

太白贝母多糖分离及结构表征

作  者：
张华;杜慧慧;冉黄桥;陶红;庞世海;罗明和;蒋永波;周浓
单  位：
关键词：
太白贝母;多糖分离;大孔吸附树脂;单糖组成;结构表征
摘  要：
利用水提醇沉得到太白贝母粗多糖之后,依次采用AB-8型大孔吸附树脂吸附、透析袋透析、二乙基氨基乙基(diethylaminoethyl, DEAE)弱碱性阴离子交换纤维柱层析对太白贝母多糖进行纯化,之后利用葡聚糖凝胶G-100柱层析法测定太白贝母多糖的相对分子质量,利用硅胶薄层层析法,气相-质谱联用法以及傅里叶红外光谱法分析研究太白贝母多糖中单糖的组成类型以及结构特点.结果表明太白贝母多糖经过DEAE纤维素柱纯化洗脱后,至少得到3个分离的洗脱峰,其中NaCl洗脱液洗脱多糖葡聚糖凝胶G-100层析柱洗脱V_e/V_o的比值为1.07;薄层层析结果显示太白贝母多糖水解物的Rf值为0.39,与D(+)-半乳糖的Rf值(0.38)迁移率接近,并且显色接近一致(均为蓝色);太白贝母多糖水解物GC-MS出峰时间(1.522 min)与D(+)-半乳糖出峰时间接近(1.520 min),并且主要m/z碎片信息接近为147.1和75.0.红外光谱特性分析研究,显示其在1 100～1 010 cm~(-1)之间有3个强吸收峰(1 050 cm~(-1 ), 1 027 cm~(-1 ), 1 003 cm~(-1 )),此为吡喃糖苷的特征吸收峰;在827 cm~(-1 )处有吸收峰,而在890 cm~(-1 )处无吸收峰,此为α-型糖苷键链接特征.可见太白贝母NaCl洗脱液洗脱的多糖相对分子质量约为2×10~6,多糖的主要组成单糖类型是D(+)-半乳糖,属于α-型吡喃糖.
译  名：
Separation and Structure Characterization of Polysaccharides in Fritillaria taipaiensis P.Y.Li
作  者：
ZHANG Hua;DU Hui-hui;RAN Huang-qiao;TAO Hong;PANG Shi-hai;LUO Ming-he;JIANG Yong-bo;ZHOU Nong;College of Biology & Food Engineering,Chongqing Three Gorges University;Engineering Technology Research Center of Characteristic Biological Resources in Northeast of Chongqing;School of Pharmaceutical Sciences,Wuhan University/Key Laboratory of Comlinatorial Biosynthesis and Drug Discovery(Ministry of Education);
关键词：
Fritillaria taipaiensis P.Y.Li;;polysaccharide separation;;macroporous adsorption resin;;monosaccharide composition;;structure characterization
摘  要：
Crude polysaccharides of Fritillaria taipaiensis P. Y. Li(Tai Bai Bei Mu) were extracted with hot water and precipitated with cold ethanol. Next, they were adsorbed with AB-8 macroporous adsorption resin, separated with dialysis bag and purified with diethylaminoethyl(DEAE) faintly alkaline anion-exchange cellulose column chromatography. Finally, Sephadex G-100 chromatography was employed to determine the relative molecular mass and thin layer chromatography(TLC), chromatography-mass spectrometer(GC-MS), and Fourier transform infrared(FTIR) spectrometer were used to analyze their composition and structure. The results showed that there were at least 3 peaks eluted by DEAE cellulose, and the V_e/V_o value of the polysaccharides eluted by NaCl elution was 1.07. TLC showed that the Rf value of polysaccharide hydrolysate of F. taipaiensis(0.39) was similar to that of D(+)-galactose(0.38), and their color was similar(blue). The GC-MS also showed that the scan time was both close to each other(1.522 min and 1.520 min) and the m/z fragment information was similar(147.1 and 75.0), respectively. The FTIR results showed 3 strong absorption peaks(1 050 cm~(-1), 1 027 cm~(-1) and 1 003 cm~(-1)) between 1 100～1 010 cm~(-1), which are the characteristic absorption peaks of pyranose, and that there was a strong absorption peak at 827 cm~(-1) and no absorption at 890 cm~(-1), suggesting an α type. In conclusion, the relative molecular mass of the polysaccharides of F. taipaiensis eluted by NaCl elution was 2×10~6, and the monosacharide included in the polysaccharides was mainly D(+)-galactose, which belongs to α-pyranose.

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