单 位：
植物-土壤相互作用教育部重点实验室农业部植物营养与养分循环重点实验室中国农业大学资源与环境学院;英国洛桑实验站农业与环境系
关键词：
遏蓝菜;Zn;超积累;超氧化物歧化酶活性
摘 要：
Zn超积累植物遏蓝菜(Thlaspi caerulescens L.)是一种珍贵的植物资源,对其体内酶活性的变化目前研究很少。采用Zn处理浓度为0、50、500、1 000 mg.kg-1的土壤培养试验和Zn处理浓度为0、50、500、1 000μmol.L-1的营养液培养试验,研究了Zn超积累植物遏蓝菜的两个生态型Prayon和Ganges地上部对Zn的积累特点及其超氧化物歧化酶(SOD)活性。结果表明:土壤培养和营养液培养条件下,随着Zn处理浓度的增加,Prayon和Ganges地上部Zn含量显著增加。其中,在土壤培养条件下,Prayon和Ganges地上部Zn含量最高分别达到4 584 mg.kg-1 DW和5 702 mg.kg-1 DW。在营养液培养条件下,Prayon和Ganges地上部Zn含量最高分别达到12 287 mg.kg-1 DW和15 966 mg.kg-1 DW。随着Zn处理浓度的增加,两个生态型叶片SOD活性显著增加。土壤培养条件下,Prayon和Ganges叶片SOD活性的变化分别是:从23.1 units.g-1 FW增加到51.2 units.g-1 FW和从23.2 units.g-1 FW增加到61.3units.g-1 FW。营养液培养条件下,Prayon和Ganges地上部SOD活性的变化分别是:从19.8 units.g-1 FW增加到44.6 units.g-1 FW和从62.4 units.g-1 FW增加到85.0 units.g-1 FW。相关分析发现两个生态型地上部Zn浓度与SOD活性极显著高度正相关。说明SOD活性的提高是Thlaspi caerulescen超积累Zn的内在机制之一。
译 名：
Effects of Zinc Amendment on Superoxide Dismutase Activity of Zinc Hyperaccumulator Thlaspi caerulescens L.
作 者：
LIU Mei-qing 1,JIANG Rong-feng 1,ZHAO Fang-jie2(1.Key Laboratory of Plant-Soil Interactons,MOE;Key Laboratory of Plant Nutrition and Nutrient Cycling,MOA;College of Resources and Environmental Sciences,China Agricultural University;Beijing 100094,China;2.Agriculture and Environment Division,Rothamsted Research,Harpenden,Hertfordshire AL5 2QJ,UK)
关键词：
Thlaspi caerulescens L.;Zn;hyperaccumulation;superoxide dismutase activity
摘 要：
Zn-hyperaccumulators have been receiving an increased attention in recent years and Thlaspi caerulescens L.is the best known example of a Zn hyperaccumulator.Soil and hydroponic experiments were carried out to investigate the effects of Zn treatments on Zn uptake and superoxide dismutase activity in two ecotypes(Prayon and Ganges) of the hyperaccumulator Thlaspi caerulescens.Zn treatments varied from 0 to 1 000 mg·kg-1 in soil experiment and from 0 to 1 000 μmol·L-1 in hydroponic experiment.The results showed that Zn uptake increased with the Zn treatment concentrations in both ecotypes.In the soil experiment,the concentration of Zn in shoots increased from 99 mg·kg-1 to 4 584 mg·kg-1 DW in Prayon and from 162 mg·kg-1 to 5 702 mg·kg-1 DW in Ganges,respectively.In the hydroponic experiment,the concentrations of Zn in the shoots increased from 1 926 mg·kg-1 to 12 287 mg·kg-1 DW in Prayon and from 3 828 mg·kg-1 to 15 966 mg·kg-1 DW in Ganges,respectively.The activities of SOD increased from 23.1 to 51.2 units·g-1 FW in Prayon and from 23.2 to 61.3 units·g-1 FW in Ganges in the soil experiment,respectively.In the hydroponic experiment,the activities of SOD increased from 19.8 to 44.6 units·g-1 FW in Prayon and from 62.4 to 85.0 units·g-1 FW in Ganges,respectively.It may be suggested that increasing activity of SOD probably play an important role for Zn hyperaccumulator Thlaspi caerulescens.
