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Position: Home > Articles > Introns Identification and Transient Expression Analysis of NtNAC-R1 Gene in Tobacco(Nicotiana tabacum L.) Journal of Henan Agricultural Sciences 2013,42 (7) 27-31

烟草NtNACR1基因内含子鉴定与瞬时表达分析

作  者:
程广伟;李桂玲;程志鹏;胡曼;刘卫群
单  位:
河南农业大学国家烟草栽培重点实验室;河南农业大学生命科学学院
关键词:
烟草;NtNAC-R1;瞬时表达分析;信号途径;内含子
摘  要:
克隆烟草NtNAC-R1基因,并进行瞬时表达分析,探讨其是否含有内含子及其在烟碱生物合成中的调控作用。以烟草基因组DNA为模板克隆得到NAC转录因子NtNAC-R1,鉴定结果表明,该基因含有2个内含子。通过构建真核表达载体,建立瞬时表达分析体系,RT-PCR检测结果显示NtNAC-R1基因过表达,烟碱生物合成关键基因PMT表达量升高,JA信号途径标记基因PDF1.2和IAA响应基因IAA13表达量降低。瞬时表达分析表明,该基因受JA信号和IAA信号途径的交互对话影响,进而调控烟碱的生物合成。
译  名:
Introns Identification and Transient Expression Analysis of NtNAC-R1 Gene in Tobacco(Nicotiana tabacum L.)
作  者:
CHENG Guang-wei1,LI Gui-ling2,CHENG Zhi-peng1,HU Man1,LIU Wei-qun1,2(1.College of Life Science,Henan Agricultural University,Zhengzhou 450002,China; 2.Key Laboratory of National Tobacco Cultivation,Henan Agricultural University,Zhengzhou 450002,China)
关键词:
Nicotiana tabacum L.;NtNAC-R1 gene;transient expression analysis;signal pathway;introns
摘  要:
Cloning and transient expression analysis of tobacco NtNAC-R1 gene was performed to discuss whether it contains introns and its function in nicotine biosynthesis.In this study,NAC transcription factor NtNAC-R1 was isolated by using tobacco genomic DNA and its two introns were identified.By constructing eukaryotic expression vector and establishing transient expression analysis system,over-expression of NtNAC-R1 gene followed by RT-PCR detection indicated that the expression amount of nicotine biosynthesis key gene PMT increased while JA signal marker gene PDF1.2 and IAA response gene IAA13 decreased.Transient expression analysis suggested that the gene regulated nicotine biosynthesis under the cross talking of JA and IAA signal pathway.

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