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Position: Home > Articles > Callus Induction and Differentiation of Dioscorea panthaica Journal of Anhui Agricultural Sciences 2007,26 (22) 140-141+144

黄山药愈伤组织的诱导与分化

作  者:
张玲;杨国涛;李海青
单  位:
西南科技大学生命科学与工程学院
关键词:
黄山药;愈伤组织诱导;体外繁殖
摘  要:
研究黄山药愈伤组织形成、器官分化和植株再生,结果表明:叶片是诱导黄山药愈伤组织的最佳外植体;含2,4-D2.0 mg/L和BA2.0 mg/L的培养基对愈伤组织诱导效果最好;大多数愈伤组织在含有1.0 mg/LBA、0.5 mg/L NAA和2%蔗糖的培养基(pH值6.4)中能诱导出芽;含有0.5 mg/LBA、0.1 mg/L NAA和3%蔗糖的MS培养基(pH值6.8)能诱导大多数转接芽中根的形成。
译  名:
Callus Induction and Differentiation of Dioscorea panthaica
作  者:
ZHANG Ling et al (School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang,Sichuan 621010)
关键词:
Dioscorea panthaica;Callus induction;In vitro propagation.
摘  要:
The callus formation,organ differentiation and plantlet regeneration of Dioscorea panthaica were studied.The results showed that leaves were the best explants for callus induction;medium with 2.0 mg/L 2,4-D and 2.0 mg/L BA was optimal for callus induction;most callus showed shoot differentiation on medium with 1.0 mg/L BA,0.5 mg/L NAA,2% sucrose and pH 6.4,and medium with 0.5 mg/L BA,0.1 mg/L NAA,3% MS sucrose and pH 6.8 allowed for root formation in most of the sub cultured shoots.

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