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Position: Home > Articles > Determination of total flavanoid in Stellera chamaejasme by synchronous fluorimetry Guangdong Agricultural Sciences 2012,39 (5) 100-101

同步荧光法测定瑞香狼毒中的总黄酮含量

作  者:
罗喜荣;李桂兰;杨军;田弋夫
单  位:
中国科学院地球化学研究所;贵阳医学院
关键词:
同步荧光法;瑞香狼毒;总黄酮
摘  要:
采用同步荧光法测定瑞香狼毒中的总黄酮含量,以芦丁为标准品,选择Δλ=50 nm,测定λ=437 nm处的同步荧光强度。结果表明,芦丁的线性范围为2.87~5 000μg/L,检出限为0.86μg/L,回收率为98.82%~100.51%,相对标准偏差为0.49%。该法操作简便、准确性好,具有良好的分析应用前景。
译  名:
Determination of total flavanoid in Stellera chamaejasme by synchronous fluorimetry
作  者:
LUO Xi-rong1,LI Gui-lan1,YANG Jun2,TIAN Yi-fu2(1.Guiyang Medical College,Guiyang 550004,China; 2.Institute of Geochemistry,Chinese Academy of Sciences,Guiyang 550002,China)
关键词:
synchronous fluorimetry;Stellera chamaejasme;total flavonoid
摘  要:
Synchronous fluorimetry was applied to assay the total flavanoid in Stellera chamaejasme.The standard sample was rutin,synchronous fluorescence intensity at λ=437 nm was determined while Δλ=50 nm.The results showed rutin linear range was 2.87~5 000 μg/L,the detection limit is 0.86 μg/L,the recovery was 98.82%~100.51%,and the relative standard deviations was 0.49%.The method was simple,accurate and had bright future in analytical applications.

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