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Position: Home > Articles > Study on Extraction Techniques of Protein from Perilla Hubei Agricultural Sciences 2013,52 (16) 3951-3953,3956

紫苏蛋白质提取工艺优化

作  者:
田海娟;刘晓莉
单  位:
攀枝花学院;吉林工商学院食品工程分院
关键词:
紫苏(Perilla frutescens);碱溶酸沉法;蛋白质提取工艺
摘  要:
以脱脂紫苏(Perilla frutescens)叶和茎为原料,采用碱溶酸沉法制备紫苏蛋白质。通过单因素试验和正交试验研究并优化紫苏蛋白质的提取工艺条件。结果表明,紫苏蛋白质的最佳制备工艺条件为碱提pH 8.0、料液比1∶10(m/V,g∶mL)、碱提温度40℃、酸沉pH 4.0,在此条件下,紫苏蛋白质提取率为20.51%;在最佳工艺条件下,采用100 W的超声波辅助提取,蛋白质提取率为22.41%。
译  名:
Study on Extraction Techniques of Protein from Perilla
作  者:
TIAN Hai-juan;LIU Xiao-li;Branch of Food Engineering,Jilin Business and Technology College;Jilin Provincial Key Laboratory of Grain and Oil Processing,Jilin Business and Technology College;Panzhihua University;
关键词:
perilla(Perilla frutescens);;alkali-extraction and acid-precipitation method;;protein extraction technology
摘  要:
Perilla(Perilla frutescens) protein was prepared by using defatted perilla leaf and stem as materiel through alkaliextraction and acid-precipitation method. The processing conditions of perilla protein were studied and optimized by single factor tests and orthogonal experiment. The results showed that the optimum extraction conditions were, pH for alkali-extraction,8.0; solid to liquid ratio, 1∶10(m / V,g∶mL); alkali-extraction temperature, 40 ℃; pH for acid-precipitation, 4.0. Under these conditions, the yield of perilla protein was 20.51%. If assisted with 100 W ultrasonic during the extracting process under optimum conditions, the yield of perilla protein was 22.41%.

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