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Position: Home > Articles > Development of Magnetic Bead-Based Chemiluminescent Enzyme Immunoassay for Chloramphenicol Detection in Swine Muscle FOOD SCIENCE 2017,38 (10) 305-309

磁性化学发光酶免疫法检测猪肉中的氯霉素

作者：

王玲;管笛;周欣;高书涛;臧晓欢;魏颖

单位：

北京市理化分析测试中心;河北农业大学理学院

关键词：

化学发光酶免疫法;氯霉素;磁性微球;猪肉

摘要：

建立以磁性微球为固定相的化学发光酶免疫法,定量检测猪肉中的氯霉素含量。此方法为在微孔中加入羊抗鼠磁性微球、样品和抗体,反应一定时间,之后补加酶标抗原,继续反应一定时间后清洗磁珠,加入发光液反应10 min后分析测定。通过磁珠用量、酶标抗原量、抗体量和反应模式的优化,成功建立了一种高灵敏度、高准确度的检测猪肉中氯霉素的磁性化学发光酶免疫方法。该方法的检出限为0.013μg/kg,线性范围在0.060~2.421μg/kg之间,回收率在85.24%~93.57%之间,相对标准偏差小于11.5%。

译名：

Development of Magnetic Bead-Based Chemiluminescent Enzyme Immunoassay for Chloramphenicol Detection in Swine Muscle

作者：

WANG Ling;GUAN Di;ZHOU Xin;GAO Shutao;ZANG Xiaohuan;WEI Ying;College of Science,Hebei Agricultural University;Beijing Center for Physical and Chemical Analysis;

关键词：

chemiluminescent enzyme immunoassay;;chloramphenicol;;magnetic beads;;swine muscle

摘要：

A chemiluminescent enzyme immunoassay based on magnetic beads was developed for quantitative determination of chloramphenicol(CAP) residues in pork samples. Sample, anti-chloramphenicol antibody, and magnetic beads coated with goat anti-mouse antibody were mixed on the microwell plate. After reacting for a certain period of time, CAP-horseradish peroxidase(HRP) was added and incubated. Then chemiluminescence substrate solution was added after washing the magnetic beads three times. Ten minutes later, the results were recorded by a microplate reader. Besides, the amount of magnetic beads coated with goat anti-mouse antibody, CAP-HRP, antibody concentration and reaction pattern were optimized. A sensitive and accurate magnetic bead-based chemiluminescent enzyme immunoassay for CAP was successfully developed. The limit of detection was 0.013 μg/kg, and good linear relationship was observed in the concentration range between 0.060 and 2.421 μg/kg. The recoveries of spiked pork samples varied from 85.24% to 93.57%, with relative standard deviation of less than 11.5%.

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磁性化学发光酶免疫法检测猪肉中的氯霉素

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