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当前位置: 首页 > 文章 > 不同竹种竹笋中5-脱氧独脚金醇的UPLC分析 核农学报 2015,29 (6) 1114-1120
Position: Home > Articles > UPLC-based Analysis of 5-deoxystrgol Extracted From Bamboo Shoots of Different Species Journal of Nuclear Agricultural Sciences 2015,29 (6) 1114-1120

不同竹种竹笋中5-脱氧独脚金醇的UPLC分析

作  者：
胡骁伟;李国栋;刘颖坤;桂仁意;张少伟;谢寅峰
单  位：
浙江农林大学/亚热带森林培育国家重点实验室培育基地;浙江省建德市林业局;南京林业大学森林资源与环境学院
关键词：
固相萃取;超高效液相色谱仪;竹笋;独脚金内酯;5-脱氧独角金醇
摘  要：
5-脱氧独角金醇(5-DS)是植物激素独脚金内酯的合成前体,是当前独脚金内酯定量的重要指标。为研究独角内脂对植物分枝/分蘖的抑制作用,本研究通过比较不同提取剂、提取纯化方法对刚竹属不同竹种竹笋5-DS含量的影响。结果发现:乙酸乙酯作为提取剂,利用超声辅助提取可节约前处理时间并提高提取率,固相萃取纯化可有效减少杂峰干扰。经以上处理后,使用超高效液相色谱仪(UPLC)能快速准确检测竹笋组织中5-DS的含量。40~8 000 ng·m L-1范围内线性关系良好,检出限为8.7 ng·m L-1,定量限为29.0 ng·m L-1;平均加标回收率在87.9%~116.5%。检测发现不同种类竹笋中5-DS含量变化为11.27~51.17 ng·g-1(FW),其中毛竹中5-DS含量最高。本研究使用超高效液相色谱仪建立竹笋中5-DS的前处理和检测方法,方法准确、精密度高、重现性好,可为竹子的分枝生理研究提供参考。
译  名：
UPLC-based Analysis of 5-deoxystrgol Extracted From Bamboo Shoots of Different Species
作  者：
HU Xiaowei;LI Guodong;LIU Yingkun;GUI Renyi;ZHANG Shaowei;XIE Yinfeng;The Nurturing Station for the State Key Laboratory of Subtropical Silviculture / Zhejiang Agriculture and Forestry University;Forestry Bureau of Jiande;Forest Resource and Environment College,Nanjing Forest University;
关键词：
solid-phase extraction,ultra performance liquid chromatography,bamboo shoots,strigolactones,5-deoxystrgol
摘  要：
5-deoxystrgol( 5-DS),a precursor of strigolactones( SLs) that are a phytohormone inhibiting shoot branching,is an essential parameter for quantification of SLs. This paper studied effects of different agents and methods for extraction and purification on the content of 5-DS isolated from bamboo shoots of different species of Phyllostchys.Results showed that ultrasonically assisted extraction with ethyl acetate could not only minimize extraction time but also raise the percentage of extraction; and solid-phase extraction and purification could effectively reduce interference from noise peaks. The 5-DS content could be detected and measured quickly and accurately from the bamboo shoot tissue pretreated with the methods mentioned above by reversed-phase ultra performance liquid chromatography( RP-UPLC).A good linear relationship for analysis was observed between 40 to 8000 ng·m L-1,the limit for detection and quantification was 8. 7 ng·m L-1and 29. 0 ng·m L-1,respectively,and an average rate of recovery with standard samples included ranged from 87. 9% to 116. 5%. It was found from measurements that the content of 5-DS from bamboo shoots of different species varied from 11. 27 to 51. 17 ng·g-1( FW),out of which that from shoots of Ph. edulis is the highest. Overall,these pre-treatment and detection methods established on the basis of RP-UPLC for detection of 5-DS from bamboo shoots were accurate, high in performance, and good in repetition, which could be used in the physiological study of branching in bamboo.

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