中国农业期刊集成服务平台

当前位置: 首页 > 文章 > 生长抑素基因初级克隆质粒(pUC12-SS)的改造与鉴定南京农业大学学报 1991,14 (3) 87-90

Position: Home > Articles > MODIFICATION AND IDENTIFICATION OF THE PRIMARY SOMATOSTATIN GENE CLONED PLASMID Journal of Nanjing Agricultural University 1991,14 (3) 87-90

生长抑素基因初级克隆质粒(pUC12-SS)的改造与鉴定

作者：

徐文忠;杜念兴;李光地;汪垣

单位：

南京农业大学兽医学系

关键词：

生长抑素基因;质粒;改造;鉴定

摘要：

生长抑素(somatostatin,ss)基因初级克隆质粒(pUC12-SS)含有限制性内切酶BamHI和HindⅢ的重复位点,用HindⅢ酶切处理和T_4DNA连接酶连接后。获得了它们的单一位点。并对经过修饰后的质粒进行了多个酶位点、SS基因片段回切和DNA序列等全面分析和证实。

译名：

MODIFICATION AND IDENTIFICATION OF THE PRIMARY SOMATOSTATIN GENE CLONED PLASMID

作者：

Xu Wenzhong, Du Nianxing,(Department of Veterinary Science. Nanjing Agricultural University)Li Guangdi and Wang Yuan(Shanghai Institute of Biochemistry, Academia Sinica)

关键词：

somatostatin gene; plasmid; modification; idcntification

摘要：

Immunization against somatostatin (SS) has been shown to stimulate the growth of animals with the increase of growth hormone in the plasma. To develop an SS vaccine with high immunogenicity, thc fusion of SS gene with hapatitis B virus antigen (HBsAg or HBcAg) gene is designed since the fusion protein can self-assemble into polymer particles which may present large amount of SS antigcnie determinants on their surfaces. This paper deais with the modification and identification of the primary SS gene cloned plasmid (pUC12-SS). The pUC12-SS with dual sites of BamH Ⅰ and HindⅢ was digested with ItindⅢ. As a result, unique cndonuclcase sites were produced for the gene fusion. Thc cndonuclcase sites related, SS gene fragment and DNA sequence were determined.

相似文章

13/17罗伯逊易位猪生长抑素基因的多态性研究 [赵雯, 姜丽花, 何晓波, 张廷荣, 孙金海] 中国畜牧兽医 2009,36 (1) 56-60
CRISPR/Cas9体外酶切检测猪生长抑素基因定点修饰靶点活性的研究 [李晓敏, 任红艳, 毕延震, 刘西梅, 郑新民] 中国畜牧兽医 2016,43 (1) 31-38
SST基因多态性及其与牛生长性状的关联分析 [高丽, 昝林森, 薛梅, 焦阳, 宋付标] 西北农林科技大学学报(自然科学版) 2011 (11) 65-70
生长抑素基因在动物育种中的应用 [杨东英, 田志环, 唐立刚, 焦传珍, 张良志] 安徽农业科学 2007,35 (33) 10731-10732+10753

导出引用

计量

文章访问数: 11

HTML全文浏览量: 0

PDF下载量: 0

生长抑素基因初级克隆质粒(pUC12-SS)的改造与鉴定

相似文章

所属期刊

南京农业大学学报

推荐期刊

西北农林科技大学学报(自然科学版)

中国畜牧兽医