Preparation and Characterization of D Type Botulinum Toxin Protein Biomimetic Mineralized Rodenticide

LI Sheng-qing;ZHANG Xi-yun;LIU Huai-xin;LI Zhi-ning;FAN Yu-xia;HUANG Rong;HU Guo-yuan;WANG Ting-ting;Qinghai Academy of Animal Science and Veterinary Medicine;State Key Laboratory of Plateau Ecology and Agriculture;

biomimetic mineralization technology;;D-type botulinum toxin;;plateau zokor;;plateau pika;;LD_(50)

[Aims] In order to expand the scope of use of D-type botulinum toxin rodenticide,exploring the biomimetic mineralization process to improve the tolerance of D-type botulinum toxin protein to environmental temperature and reduce the destruction intensity of gastrointestinal contents of toxins.[Methods] The advanced biomimetic mineralization technology was used to protect the botulinum toxin type D,and the process flow of the D-type botulinum toxin biomimetic granule was screened.The thermal stability and oral lethality(LD_(50)) of mineralized toxin particles at different temperatures were determined by biological methods.[Results] The shelf life of mineralized samples of botulinum toxin type D was significantly longer than that of the control group,the toxicity loss was significantly reduced,and the resistance of mineralized toxin to the destruction intensity of intestinal contents was significantly enhanced.The minimum lethal dose of mineralized toxin particles to plateau zokor,plateau pika and mice was 0.52 ×10~4,0.86 ×10~4 and 0.25 ×10~4 MLD/kg,respectively.The lethality and palatability of mineralized toxin particles to the pests were good.[Conclusions] The biomimetic mineralization technology can greatly improve the thermal stability of botulinum toxin type D and reduce the damage of gastrointestinal contents to botulinum toxin type D.The mineralized toxin can be used not only for the population density of grassland rodent,and can be extended to the prevention and treatment of urban rodent pests.At the same time,the research provides a new idea for the protection of toxin protein substances.