关键词：
褐牙鲆;钙、镁浓度;生长;SOD和CAT酶活力
摘 要：
在盐度30和水温20℃条件下,配制钙、镁(1∶3)总浓度A(700mg/L)、B(1100mg/L)、C(1600mg/L,对照)、D(2200mg/L)、E(2800mg/L)的人工海水,研究了5种人工海水对褐牙鲆Paralichthys olivaceus幼鱼生长、肝脏超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力的影响。观察发现,褐牙鲆幼鱼初次接触高钙、镁浓度人工海水(D组和E组)时产生应激反应,鱼体呈弓形,呼吸频率降低,摄食不积极,适应7d左右恢复正常。经60d养殖实验,各处理组实验鱼食物转化效率(FCE)无显著差异(P>0.05),成活率均在90%以上。0~20d时实验鱼特定生长率(SGR)和摄食率(FI)差异显著(P<0.05),低浓度组大于高浓度组,20d后,FI无显著差异(P>0.05),40~60d时低浓度组SGR小于高浓度组。各处理组SGR和FI随钙、镁总浓度上升呈下降趋势,实验进行到60d时,低浓度组A的SGR和FI分别为高浓度组E的1.13倍和1.04倍。不同钙、镁浓度对实验鱼的免疫酶活性亦有显著影响。D组实验鱼SOD酶活力和肝比重显著高于其他各处理组;E组SOD酶活力显著低于其他实验组(P<0.05),CAT酶活力也低于其他处理组,但各组间差异不显著(P>0.05)。研究结果表明,实验初期钙、镁浓度通过影响褐牙鲆的摄食而影响其生长,低钙、镁浓度组实验鱼生长较快,高浓度组生长较慢。经60d养殖驯化,除E组外的各处理组特定生长率差异不显著,过高浓度钙、镁对褐牙鲆的免疫酶活性产生一定抑制作用。建议养殖褐牙鲆时应注意避免水体钙、镁含量过高。
译 名：
Effects of Ca~(2+) and Mg~(2+) concentrations on growth,SOD and CAT enzymatic activity of juvenile Paralichthys olivaceus
作 者：
WANG Mao-lin1,2 ZHANG Xiu-mei2 HUANG Guo-qiang2ZHANG Pei-dong2 LI Jun-feng1(1Key Laboratory of Mariculture,Ministry of Agriculture,Dalian Ocean University,116023)(2Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao 266003)
关键词：
Paralichthys olivaceus Calcium and magnesium concentrations Growth SOD CAT
摘 要：
The effects of Ca2+ and Mg2+ concentrations(1∶3)in artificial seawater on growth,superoxidase dismutase(SOD)and catalase(CAT)enzymatic activity of juvenile Paralichthys olivaceus were investigated at 20℃ and salinity of 30.Five concentrations of Ca2+ and Mg2+ were set as follows,700(group A),1 100(group B),1 600(group C),2 200(group D),and 2 800(group E)mg/L,respectively.It was found that stress response occurred including bend of fish body and reduction of ventilation rate and food intake in juvenile fish under high Ca2+ and Mg2+ concentrations and the fish recovered after a week.After 60 d experiment,the survival rates of all groups were over 90%,and there were no significant differences in food conversion efficiencies(FCE)among different groups(P>0.05).However,the effects of Ca2+ and Mg2+ concentrations in artificial seawater on specific growth rates(SGR)and food intake(FI)at 0~20 d were significant(P<0.05).After 20 d,there was no significant difference in FI(P>0.05).During 40~60 d,the SGRs in low Ca2+ and Mg2+ concentrations groups were lower than that in high concentrations groups.The SGR and FI of all groups decreased when Ca2+ and Mg2+ concentrations increased.At 60 d,the SGR and FI of group A were 1.13 and 1.04 times as that of group E,respectively.Significant influences were also observed in immuno-enzymatic activity of liver among these groups(P<0.05).The activity of SOD and hepatosomatic index of group D were significantly higher than those of other groups.However,the activity of SOD of group E was lower than other groups.The activity of CAT of group E was also lower than other groups but no significant difference(P>0.05).The results indicated that the concentrations of Ca2+ and Mg2+hindered the growth of juvenile P.olivaceus by changing their food intake.The fish in low Ca2+ and Mg2+ concentration groups grew fast and those in high Ca2+ and Mg2+ concentration groups grew slowly.There were no significant differences in the SGR of all the groups except for group E after 60 d rearing experiment.High Ca2+ and Mg2+ concentrations could restrain immuno-enzymatic activity of juvenile P.olivaceus.It is suggested that high concentration of Ca2+ and Mg2+ should be avoided in the culturing of juvenile P.olivaceus.
