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Position: Home > Articles > Preliminary selection for cotton cultivar transformed with Trichoderma chitinase gene in Xinjiang upland cotton Xinjiang Agricultural Sciences 2005,42 (3) 178-180

新疆陆地棉转木霉几丁质酶基因棉花的初步筛选

作  者:
李莉;曲延英;陈全家;夏瑜华;兰杰;尤春源
单  位:
新疆农业大学农学院
关键词:
陆地棉;转基因;花粉管通道法;选择标记
摘  要:
利用花粉管通道法将构建在植物表达载体pART27上的抗黄萎病基因木霉几丁质酶(Trichodermachi-tinase)基因,导入新疆4个陆地棉品种(系)中,经标记基因阳性试验和病圃抗病性检测,获得T2代具有较高的抗病性植株30株。
译  名:
Preliminary selection for cotton cultivar transformed with Trichoderma chitinase gene in Xinjiang upland cotton
作  者:
LI Li,QU Yan-ying,Chen Quan-jia,XIA Yu-hua,LAN Jie,YOU Chun-yuan (College of Agronomy, Xinjiang Agricultural University, Urumqi830052,China)
关键词:
upland cotton; transfering gene; pollen tube pathway; selection marker
摘  要:
Trichoderma chitinase gene in plant expression vector, pART27, was transformed into 4 cultivars(lines) of Xinjiang upland cotton by pollen-tube pathway method. 30 Trichoderma chitinase gene-transgenic in T_2 plants with satisfactory resistance to verticillum wilt was obtained through selectable marker gene identified as positive strain and cotton-resistant check.

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