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当前位置: 首页 > 文章 > 鸡肝脏和肌肉组织中常山酮残留的ELISA检测 畜牧兽医学报 2006,37 (6) 597-602
Position: Home > Articles > Detection of Halofuginone in Chicken Liver and Chicken Muscle by Enzyme Linked ImmunoSorbent Assay Chinese Journal of Animal and Veterinary Sciences 2006,37 (6) 597-602

鸡肝脏和肌肉组织中常山酮残留的ELISA检测

作  者：
吴宁鹏;杜向党;沈建忠
单  位：
中国农业大学动物医学院
关键词：
常山酮;鸡肝脏;鸡肌肉;残留;酶联免疫吸附测定法
摘  要：
将常山酮进行人工改造,制备了半抗原常山酮琥珀酸衍生物(Hal-suc)。采用N-羟基琥珀酰亚胺活性酯法将半抗原与牛血清白蛋白(BSA)、卵清白蛋白(OVA)偶联,制备免疫原和包被原。动物免疫6次后采血制备抗血清,以间接ELISA法测定血清效价,测得抗血清的最佳工作浓度为1∶102 400。建立了常山酮在鸡肝脏和肌肉组织中检测的ELISA法,该方法在50、100、500 ng/g的添加浓度水平下,在鸡肝脏和肌肉组织中测得的平均回收率范围分别为74.2%~96.8%和74.3%~90.0%,检测限分别为28和19 ng/g。
译  名：
Detection of Halofuginone in Chicken Liver and Chicken Muscle by Enzyme Linked ImmunoSorbent Assay
作  者：
WU Ning-peng~(1,2),DU Xiang-dang~1,SHEN Jian-zhong~(1*)(1.College of Veterinary Medicine,China Agricultural University,Beijing 100094,China;2.Henan Provincial Supervising Agency of Veterinary Drugs,Zhengzhou 450008,China)
关键词：
halofuginone;chicken liver;chicken muscle;residues; enzyme-linked immunosorbent assay(ELISA)
摘  要：
The halofuginone was modified to prepare Hal-suc as hapten.The hapten was conjugated to BSA as immunogen and to OVA as coated antigen by NHS ester procedure.The anti-Hal serum was acquired in rabbits against the conjugate after sixth immunization.The optional dilution of the anti-Hal serum was 1:102 400 by the indirect ELISA.An ELISA method for the determination of the Hal in chicken liver and muscle tissues was developed.In the chicken liver tissue fortified at 50,100,500 ng/g,the average recoveries ranged from 74.2% to 96.8%.In the chicken muscle tissue fortified at 50,100,500ng/g,the average recoveries ranged from 74.3% to 90.0%.The detection limits in chicken liver tissue and in chicken muscle tissue were 28ng/g and 19ng/g respectively.

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