Screening of anti-tumor active substances from Isochrysis Galbana by the method of kinase inhibitors

GUO Xiao xue,WANG Xue qing*,WANG Ying,ZHAO Pei (Tianjin Key Laboratory of Food and Biotechnology,College of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134,China)

protein tyrosine kinases(PTKs);kinase inhibitors;Isochrysis Galbana;anti tumour bioactive substances

Protein tyrosine kinases(PTKs) plays an important role in signal transduction pathways,which had become the interesting targets of screening new antitumor bioactive substances. The activity of PTKs of the fractions extracted from Isochrysis Galbana by ethyl acetate,acetone,pentyl alcohol and water were conducted respectively,for screening effective PTKs inhibitors to develop the leading compounds of antitumor drugs. The result showed the pentyl alcohol extract of Isochrysis Galbana had obvious inhibition on PTKs in treated samples,which inhibition rate was 38. 94%. And the isolated component that the value of Rf was 0. 6610 from the pentyl alcohol extract by thin layer chromatography used acetone petroleum ether(1∶ 60,v/v) as developer,had inhibition on PTKs,and inhibition rate was 32. 21%. Furthermore,by means of silica gel column chromatography which acetone was used for eluent,the acetone elution part from the former component exhibited the strongest inhibition against PTKs,and inhibition rate was 86. 09%. Moreover,the substance also represented strong cytotoxin active. Therefore,it would be the aim active substances of Isochrysis Galbana. Meanwhile,the method of kinase inhibitors is more convenient and sensitive in operation than that of cytotoxin active,and it is an attractively potential model of high throughput screening anti tumour activity substances.