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Position: Home > Articles > Polymorphism of FoxO1 gene and associations with reproduction traits in Holstein cattle of China Journal of Northeast Agricultural University 2013,44 (6) 54-57

中国荷斯坦奶牛FoxO1基因多态性及与繁殖性状关系研究

作  者:
赵佳强;周妍;于倩楠;陶林林;张淑君;易建明;滕茂平;李丛远;张军伟
单  位:
华中农业大学动物科技学院;武汉惠尔康扬子江乳业有限公司
关键词:
中国荷斯坦奶牛;SNPs;FoxO1;繁殖性状
摘  要:
通过PCR-单链构象多态性(PCR-SSCP)结合测序技术发现FoxO1第4外显子一个新单核苷酸多态性(SNP),限制性片段长度多态性聚合酶链反应(PCR-RFLP)技术分别分析925头中国荷斯坦奶牛FoxO1该位点多态性。结果表明,在中国荷斯坦奶牛中,FoxO1基因有3个基因型,分别是CC、TT、CT。其中等位基因C频率为0.95,是优势等位基因;χ2适合性检验表明该位点不符合哈代温伯格平衡(P<0.05)。该基因不同基因型对奶牛产犊间隔、空怀期以及初产日龄有显著影响(P<0.05),而对产犊到发情、犊牛初生重、配种次数影响不显著(P>0.05)。
译  名:
Polymorphism of FoxO1 gene and associations with reproduction traits in Holstein cattle of China
作  者:
ZHAO Jiaqiang1,ZHOU Yan1,YU Qiannan1,TAO Linlin1,ZHANG Shujun1,YI Jianming1,TENG Maoping2,LI Congyuan2,ZHANG Junwei2(1.Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction of Ministry of Education,School of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;2.Wuhan Hui'erkang-Yangzijiang Dairy Co.,Ltd.,Wuhan 430010,China)
关键词:
Holstein cattle of China;SNPs;FoxO1;reproduction
摘  要:
PCR-single-strand conformation polymorphism(PCR-SSCP) and sequencing are used and a novel single nucleotide polymorphisms(SNP)was detected in the forth exon of FoxO1 gene.The gene types of the 925 Holsteins were differentiated by PCR-restriction fragment length polymorphism(PCRRFLP).The result showed that there were three genotypes(CC,CT,TT) and the frequency of allele C which was superior gene was 0.95;χ2 fitness test showed that the loci deviates from the Hardy-Weinberg equilibrium(P<0.05).The correlation analysis revealed that these polymorphisms had a extremely significant effects on calving interval,open days and the first calving age(P<0.05),but there was not a significant difference with the days between calving and oestrus,the times of service per conception and birth weight(P>0.05).

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