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两种提取红松种子蛋白质的工艺方法比较研究

作  者:
吴晓红;郑月明;付兆龙;刘琴;张秀男;王振宇
单  位:
东北林业大学
关键词:
红松种子;蛋白质提取率;比较研究
摘  要:
为寻找新的植物蛋白资源,研究发现,红松种子中蛋白质含量在13%~20%,具有明显的生理活性,是一种新的具有活性的植物蛋白质资源。以脱脂红松种子粉为原料,比较PBS-NaCl缓冲液盐溶法和NaOH碱溶法提取红松种子蛋白质工艺。分别通过单因素和正交试验,确定最佳的提取工艺:研究了料液比、提取温度、提取时间、溶液浓度对蛋白质提取率的影响,并进行了L9(45)的正交试验,并测定了两种工艺得到的红松种子蛋白的等电点。PBS-NaCl缓冲液盐溶法得到的红松种子蛋白等电点pI=3.7,碱溶法提取蛋白的等电点pI=4.0。PBS-NaCl缓冲液盐溶法提取红松种子蛋白质的提取工艺:在温度30℃水浴环境下,以0.12mol/L PBS-NaCl缓冲液盐溶液提取,按1∶25的料液比搅拌浸提150min,蛋白提取率达到62.86%。碱溶法提取红松种子蛋白质的最佳工艺参数是以0.0005mol/LNaOH溶液,按1∶30料液比,在45℃条件下低速搅拌浸提60min,此时蛋白的提取率为72.07%。结论:从蛋白质提取率的角度考虑,碱溶法优于PBS-NaCl缓冲液盐溶法;从蛋白质稳定性和提取率两方面考虑,PBS-NaCl缓冲液盐溶法优于碱溶法。
译  名:
Comparative Study on Two Methods of Pine Seed Protein Extraction Technology
作  者:
Wu Xiaohong1,Zheng Yueming1,Fu Zhaolong1,Liu Qin1,Zhang Xiunan1,Wang Zhenyu1,2(1.Northeast Forestry University,Harbin 150040;2.Harbin Institute of Technology,Harbin 150001)
关键词:
Pine seed;Protein extraction rate;Comparative study
摘  要:
This paper aimed at comprehensive utilization of pine,comparative study on two methods of pine seed protein extraction technology by PBS-NaCl and NaOH solution.and the effects of solid-liquid ratio,temperature,time and concentration of solution were investigated,On the basis of single factor and the orthogonal test,the optimal working parameterts were worked out.The optimal extracting of PBS-NaCl solution conditions of protein were:solid-liquid ratio was 1∶25,30℃,0.12mol/L and extraction for 150min,the extraction rate reaches 62.86%.The optimal extracting of NaOH solution conditions of protein were:solid-liquid ratio was 1∶30,45℃,0.0005mol/L and extraction for 60min,the extraction rate reaches 72.07%.Conclusion:consideration of protein extraction rate the solution of PBS-NaCl was better than NaOH solution;From the extraction of protein and the stability of protein,the solution of PBS-NaCl was best.

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