中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > “新吉富”罗非鱼选育F_(10)～F_(13)遗传变异微卫星分析 上海海洋大学学报 2011,20 (1) 1-7
Position: Home > Articles > Genetic variations among the recent selected generations F_(10)-F_(13) of NEW GIFT strain Nile tilapia (Oreochromis niloticus) by SSR analysis Journal of Shanghai Ocean University 2011,20 (1) 1-7

“新吉富”罗非鱼选育F_(10)～F_(13)遗传变异微卫星分析

作  者：
于爱清;李思发;蔡完其
单  位：
上海海洋大学农业部水产种质资源与利用重点开放实验室
关键词：
新吉富罗非鱼;选育世代;遗传变异
摘  要：
利用微卫星分子标记技术,对"新吉富"罗非鱼选育后期的F10、F11、F12、F13 4个世代群体的遗传结构及异同进行了分析。结果表明:13个微卫星位点扩增后,共得到78个等位基因,扩增片段长度大小在100～295bp之间,4个选育世代的平均有效等位基因数(Ne)为2.84～3.27,平均期望杂合度(He)为0.627 1～0.676 2,平均多态信息含量(CPI)为0.567 6～0.622 9,3个遗传参数都一致呈现随着选育世代数累进而稳定下降的趋势,表明"新吉富"罗非鱼在长期选育过程里越来越趋于纯化之中。另一方面,4个选育世代两两间遗传分化FST值为0.019 07～0.133 54,世代间亦存在中等程度的遗传分化,显示"新吉富"罗非鱼的选育技术路线(黄河、长江及珠江3大农业生态区同步选育、适时交流,始终注意保持繁育群体的有效大小,严格的择优四步程序等)可使选育群体保持较丰富的遗传变异水平,有利于长期选育的持续开展。
译  名：
Genetic variations among the recent selected generations F_(10)-F_(13) of NEW GIFT strain Nile tilapia (Oreochromis niloticus) by SSR analysis
作  者：
YU Ai-qing,LI Si-fa,CAI Wan-qi(The Key Laboratory of Aquatic Genetic Resource and Utilization Certificated by the Ministry of Agriculture,Shanghai Ocean University,Shanghai 201306,China)
关键词：
NEW GIFT Nile tilapia strain(Oreochromis niloticus);selective breeding generation;genetic variation
摘  要：
Simple Sequence Repeats(SSR)marker analysis was used to measure the genetic structure and genetic differentiation of four successively selected generations(F10,F11,F12,F13)of NEW GIFT strain Nile tilapia(Oreochromis niloticus).The results showed that: total of 78 alleles(100-295 bp)were found among the four populations of 120 individuals with 13 polymorphic microsatellite loci.The mean number of effective alleles of four generations ranged from 2.84 to 3.27,the average polymorphism information content and the expected value of mean heterozygosity varied from 0.567 6 to 0.622 9 and from 0.627 1 to 0.676 2 in four selected generations,respectively.Three genetic parameters all indicated the same tendency that they have been steadily decreasing with the increasing of selected generations,which proved that the purifying experience of NEW GIFT strain Nile tilapia(Oreochromis niloticus)was still continuing in the course of long-term selective breeding.On the other hand,pair-wise FST values between the two arbitrary generations ranged from 0.019 07 to 0.133 54,which indicated that the selective breeding generations also generated moderate genetic differentiation.The above results showed that our applied breeding technique strategy(synchronized selection at Yellow River,Yangtze River and Pearl River ecosystems,exchange periodically,keep effective breeding number,as well as strict super-fish four-steps selection proceeding)for the NEW GIFT strain Nile tilapia(Oreochromis niloticus)could enable the selective breeding populations to maintain abundant genetic variation,which would be beneficial to the sustainable development of long-term selective breeding.

相似文章

导出引用
计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46