单 位：
云南省肉牛和牧草研究中心;云南农业大学动物科学技术学院;云南蒙自市畜牧技术推广站;云南省草地动物科学研究院
关键词：
滇东南水牛;mtDNA D-loop;遗传多样性;系统地位;沼泽型水牛
摘 要：
【目的】深入了解滇东南水牛(Bubalus bubalis)的遗传多样性及其在水牛中的系统地位。【方法】以29头滇东南水牛血样为材料,通过PCR扩增直接测序,测定了29条滇东南水牛个体的线粒体DNA D-loop(mtDNAD-loop)全序列,并引用GenBank公布的埃及、印度、地中海、巴西及中国水牛mtDNA D-loop序列,构建各单倍型间的聚类树。【结果】滇东南水牛mtDNA D-loop全序列长度为910～917bp,结合GenBank中公布的5条来自云南省马关县的滇东南水牛序列,界定滇东南水牛mtDNA D-loop有单倍型20种,单倍型多态度、核苷酸多态度和核苷酸差异数分别为0.877±0.053,0.011±0.005和9.865±21.419,错配分布分析表明,滇东南水牛保持着较为平稳的群体水平,揭示滇东南水牛现生群体的遗传多样性较为丰富。聚类结果显示,滇东南水牛所有个体都聚在沼泽型水牛支系,与印度、埃及、地中海等江河型水牛支系截然分列于2大独立的支系;滇东南水牛又分为支系A(SW-A)和支系B(SW-B),与其他中国水牛品种的表现一致。【结论】滇东南水牛群体规模平稳,且群体未曾受到印度水牛基因的渐渗,完全属于沼泽型水牛,支持滇东南水牛起源于中国或东南亚的假说。
译 名：
Genetic diversity and phylogenetic status of mitochondrial DNA control region in Diandongnan buffalo
作 者：
QU Kai-xing1,2,HE Zhan-xing2,MIAO Yong-wang3,LI Hong-wei4, JIN Xian-dong2,ZHANG Ji-cai2,YANG Guo-rong2, HUANG Bi-zhi2,ZAN Lin-sen1,5 (1 College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100,China; 2 Yunnan Academy of Grassland and Animal Science,Kunming,Yunnan 650212,China; 3 College of Animal Science and Technology,Yunnan Agricultural University,Kunming,Yunnan 650201,China; 4 Mengzi Center of Animal Disease Control and Prevention,Mengzi,Yunnan 661100,China; 5 National Beef Cattle Improvement Center of China,Yangling,Shaanxi 712100,China)
关键词：
Diandongnan buffalo;mtDNA D-loop;genetic diversity;phylogenetic status;swamp-type buffalo
摘 要：
【Objective】 The aim of this study is to shed light on the genetic diversity and phylogenetic status of Diandongnan buffalo(Bubalus bubalis).【Method】 Through polymerase chain reaction(PCR) and direct sequence,29 mtDNA D-loops were sequenced.A NJ tree was constructed by gathering the mtDNA D-loop sequences involving in Egyptian,Indian,Mediterranean,Brazilian,and Chinese buffalo breeds.【Result】 The lengths of mtDNA D-loops ranged from 910-917 bp.Combined with five mtDNA D-loops of Diandongnan buffalo from GenBank,20 haplotypes were defined showing that haplotype diversity,nucleotide diversity and average number of nucleotide differences were 0.877±0.053,0.011±0.005 and 9.865±21.419,respectively.Mismatch distribution analysis showed that extant Diandongnan buffalo population lasts a stable population with abundant diversity.The NJ tree revealed two distinct clades(swamp-type and river-type clades),and all the Diandongnan buffalo clustered together with swamp-type clade and split from river-type buffalo including Indian,Egyptian and Mediterranean buffalo.Diandongnan buffalo further split two sub-clades,SW-A and SW-B in accordance with the cases of other Chinese buffalo breeds.【Conclusion】 Diandongnan buffalo keep a stable population size and no Indian river-type mitochondrial haplotypes were found in Diandongnan buffalo indicating that Diandongnan buffalo hadn't yet been undergone gene introgression from Indian buffalo and Diandongnan buffalo belongs to pure swamp-type without Indian river-type haplotypes supporting the hypothesis of China or Southeast Asian origin.
