Position: Home > Articles > Analysis of 6 Synthetic Sweeteners in Foods by Liquid Chromatography-Quadrupole-Time-of-Flight Mass Spectrometry
FOOD SCIENCE
2014,35
(8)
181-186
液相色谱-四极杆-飞行时间质谱法筛查食品中6种人工合成甜味剂
作 者：
王建华;杨君;刘琳;颜冬云;李立;刘靖靖
单 位：
中国检验检疫科学研究院;青岛大学化学科学与工程学院(环境科学与工程学院);山东出入境检验检疫局检验检疫技术中心
关键词：
液相色谱-四极杆飞行时间串联质谱;人工合成甜味剂;筛查;食品
摘 要：
应用高效液相色谱-四极杆-飞行时间质谱建立固体样品中甜味剂的筛查。样品经前处理后采用Eclispe XDB-C18色谱柱,以0.1%甲酸-10 mmol/L甲酸铵溶液-甲醇为流动相,梯度洗脱,电喷雾负离子模式分析检测。在全扫描采集模式下,以准分子离子峰的峰面积定量,以化合物精确分子质量定性。各化合物在0.02~2.0 mg/L范围内均呈现良好的线性关系,相关系数均大于0.998。样品平均添加回收率为74.5%~120.1%,相对标准偏差均小于10.4%。本方法可满足现行法规的限量要求。
译 名：
Analysis of 6 Synthetic Sweeteners in Foods by Liquid Chromatography-Quadrupole-Time-of-Flight Mass Spectrometry
作 者：
WANG Jian-hua;YANG Jun;LIU Lin;YAN Dong-yun;LI Li;LIU Jing-jing;Technical Center of Inspection and Quarantine, Shandong Entry-Exit Inspection Quarantine Bureau;College of Chemical Engineering, Qingdao University;Chinese Academy of Inspection and Quarantine;
关键词：
liquid chromatography quadrupole-time of flight mass spectrometry;;artificial sweeteners;;screening;;food
摘 要：
A new method was established for the determination of 6 synthetic sweeteners(sodium saccharin, cyclamate, acesulfame-K, aspartame, neotame, and sucralose) in food samples by using liquid chromatography-quadrupole-time of flight mass spectrometry(LC-Q-TOF-MS). The extract was measured directly by LC-Q-TOF-MS with electrospray ionization in the negative mode. The compounds were separated by an Eclispe XDB-C18 column with acetonitril-10 mmol/L ammonium formate-0.1% formic acid as the mobile phase. A detailed fragmentation study for these 6 synthentic sweeteners was carried out by time-of-flight. The 6 compounds behaved linearly in the concentration range of 0.02–2.0 mg/L, with a correlation coefficient > 0.998. The recoveries were 74.5%–120.1%, with rela tive standard deviation below 10.4%. The method illustrated is suitable for routine qualitative and quantitative analyses of synthetic sweeteners in food samples.
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