中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 鸡胚成纤维细胞感染马立克氏病病毒后部分染色体上微卫星不稳定性研究 新疆农业大学学报 2009,32 (1) 12-17
Position: Home > Articles > Study on Microsatellite Instability on Some Chromosomes of MDV-infected Chicken Embryo Fibroblasts Journal of Xinjiang Agricultural University 2009,32 (1) 12-17

鸡胚成纤维细胞感染马立克氏病病毒后部分染色体上微卫星不稳定性研究

作  者：
高喜梅;邱燕;韩涛;姚刚;杨德吉
单  位：
新疆农业大学;南京农业大学
关键词：
鸡胚成纤维细胞;马立克氏病毒;微卫星不稳定性
摘  要：
研究鸡胚成纤维细胞(CEF)感染马立克氏病毒(MDV)后部分染色体(5,6,7,8,9)微卫星的变化。CEF感染马立克氏病超强毒RB1B株后,提取正常鸡胚成纤维细胞和感染病毒的成纤维细胞DNA,应用5,6,7,8,9号染色体上的43个微卫星引物进行PCR扩增,产物采用变性聚丙烯酰胺凝胶电泳银染检测。结果表明43个微卫星标记有38个表现出不同程度的微卫星不稳定性,ABR0262发生率最高,为38.10%,ABR0048,ABR0041和MCW0183也较高,为33.33%。21组样品中全部检测到微卫星不稳定性,9号样品微卫星不稳定性发生率最高,为41.86%。11号样品的发生率也较高,为32.56%。表明马立克氏病病毒感染鸡胚成纤维细胞后,可以导致广泛的宿主基因组变化。这些变化是随机的,但是也有一定的规律,而且这些变化可以用微卫星标记来检测。
译  名：
Study on Microsatellite Instability on Some Chromosomes of MDV-infected Chicken Embryo Fibroblasts
作  者：
GAO Xi-mei1,2,QIU Yan2,HAN Tao2,YAO Gang1,YANG De-ji2(1.College of Animal Medicine,Xinjiang Agricultural University,Urumqi,Xinjiang,830052,China;2.College of Animal Medicine,Nanjing Agricultural University,Nanjing,Jiangsu,210095,China)
关键词：
chicken embryo fibroblast;Marek's disease virus;microsatellite instability
摘  要：
Microsatellite instability(MSI) was researched on some loci of chromosomes(5,6,7,8 and 9) from chicken embryo fibroblasts(CEF)infected by Marek's disease virus(MDV) with the virulent RB1B strain.Genomic DNA of infected CEF and cells before infection was extracted,respectively.MSI of chromosome(5,6,7,8 and 9) were detected at 43 microsatellite loci by means of PCR,PAGE electrophoresis and silver staining in matched normal CEF and MDV-infected CEF.MSI were found on 38 of 43 loci,the incidence rate of ABR0262 was 38.10% and that of ABR0048.Both ABR0041,MCW0183 were 33.33%,MSI was found in all samples,the incidence rate of sample 9 was up to 41.86%,and the incidence rate of sample 11 was up to 32.56%.The results showed that MSI was found on some chromosomes of CEF infected with MDV.When infecting CEF,MDV can lead to extensive changes in the host genome.These changes are random,but regular to a certain extent,which can be detected by microsatellite markers.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46