中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 日本结缕草ZjLEA3基因克隆、亚细胞定位及表达分析 分子植物育种 2018 (13) 4219-4225
Position: Home > Articles > Cloning, Subcellular Localization and Expression Analysis of ZjLEA3 Gene in Zoysia japonica Molecular Plant Breeding 2018 (13) 4219-4225

日本结缕草ZjLEA3基因克隆、亚细胞定位及表达分析

作  者：
刘海金;滕菲;靳飞;杨青青;罗红松
关键词：
日本结缕草;LEA基因;克隆;亚细胞定位;表达分析
摘  要：
为了研究日本结缕草抗逆机制,本研究以日本结缕草(Zoysia japonica)转录组的数据为基础,克隆得到ZjLEA3基因,其开放阅读框为555 bp,编码184个氨基酸。ZjLEA3基因包含LEA基因族保守区域,与其他植物LEA基因相似度在65.79%以上。软件分析表明该蛋白属于小分子亲水性蛋白;亚细胞定位结果显示该基因编码的蛋白定位于细胞质和细胞核中;ZjLEA3表达分析表明,该基因是受到了高温、高盐、PEG的诱导,由此推断ZjLEA3可能参与非生物胁迫的防御。本试验克隆并分析ZjLEA3基因是为日本结缕草抗逆机理研究提供帮助。
译  名：
Cloning, Subcellular Localization and Expression Analysis of ZjLEA3 Gene in Zoysia japonica
作  者：
Liu Haijin;Teng Fei;Jin Fei;Yang Qingqing;Luo Hongsong;Affiliated Middle School of Beijing College of Education;Beijing Institute of Education;Beijing Forestry University;
单  位：
Liu Haijin%Teng Fei%Jin Fei%Yang Qingqing%Luo Hongsong%Affiliated Middle School of Beijing College of Education%Beijing Institute of Education%Beijing Forestry University
关键词：
Zoysia japonica;;LEA gene;;Clone;;Subcellular localization;;Expression analysis
摘  要：
In order to investigate the resistance mechanism of Zoysia japonica, ZjLEA3 gene was cloned based on the data from the Zoysia japonica transcriptome. Its open reading frame was 555 bp and encoded 184 amino acids.The ZjLEA3 gene contained a conserved region of the LEA gene family with a similarity of 65.79% or more with other plant LEA genes. Software analysis showed that the protein belonged to the small molecule hydrophilic protein; subcellular localization results indicated that the protein encoded by the gene was located in the cytoplasm and nucleus. ZjLEA3 expression analysis implied that the gene was induced by high temperature, high salt and polyethylene glycol(PEG), suggesting that ZjLEA3 might be involved in abiotic stress defense. This study cloned and analyzed ZjLEA3 gene to provide a theoretical basis for the study on Zoysia japonica resistance mechanism.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46