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当前位置: 首页 > 文章 > 莱克多巴胺人工抗原及抗体的制备 食品科学 2009,30 (9) 25-28
Position: Home > Articles > Preparation of Ractopamine Artificial Antigen and Antibody FOOD SCIENCE 2009,30 (9) 25-28

莱克多巴胺人工抗原及抗体的制备

作  者：
孙海新;凌红丽;曹照真;刘围;高亚东;郗日沫
单  位：
青岛康地恩实业有限公司;山东大学化学与化工学院
关键词：
莱克多巴胺;ELISA;偶联;免疫原
摘  要：
莱克多巴胺是一种禁用β-兴奋剂,建立莱克多巴胺药残的快速检测方法是实现对其进行有力监控的有效途径,而莱克多巴胺抗体是快速免疫检测法的基本试剂。本实验用全新的方法研究了莱克多巴胺免疫原的合成,采用对氨基苯甲酸(ABA)和1,4-丁二醇缩水甘油醚(BDE)将莱克多巴胺分别和cBSA、cOVA偶联,合成了莱克多巴胺的免疫原和包被抗原,并对其进行了紫外分析。用免疫原免疫新西兰大白兔,获得了高灵敏度和特异性的莱克多巴胺多克隆抗体,采用间接ELISA法检测抗体的IC50值为4.34ng/ml,所得多克隆抗体效价达到102400。
译  名：
Preparation of Ractopamine Artificial Antigen and Antibody
作  者：
SUN Hai-xin1,LING Hong-li1,CAO Zhao-zhen2,LIU Wei2,GAO Ya-dong1,XI Ri-mo2, (1.Qingdao Contient Industry Co.Ltd., Qingdao 266111, China; 2.School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China)
关键词：
ractopamine;ELISA;conjugation;antigen
摘  要：
As one of β-agonists, ractopamine has been prohibited in food production and animal husbandry. To enforce regulation, it is necessary to develop a rapid detection system to measure the residue of ractopamine in animal food production. In this study, a new ractopamine immunogen was synthesized by using 4-aminobenzoic acid as a linker to connect hapten with carrier protein, bovine serum albumin (BSA). Besides the immunogen, a new ractopamine coating antigen was synthesized also by using 1,4-butanediol diglycidyl ether (BDE) as a linker to connect ovalbumin (OVA). The BSA conjugate was used to immunize New Zealand rabbits and polyclonal antibodies against ractopamine were obtained. The titers of antibodies were up to 102400 by indirect ELISA and the antibodies showed high sensitivity in the heterologous assay using RAC-BDE-cOVA as coating antigen, with an IC50 value of 4.34 ng/ml. The artificial antigen has perfect immunogencity and the obtained antibody has excellent sensitivity.

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