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当前位置: 首页 > 文章 > 变换波长法测定复方樟脑搽剂中樟脑和苯酚的含量 中兽医医药杂志 2017 (2) 42-44
Position: Home > Articles > Determination of camphor and phenol in compound camphor liniment by wavelength conversion HPLC Journal of Traditional Chinese Veterinary Medicine 2017 (2) 42-44

变换波长法测定复方樟脑搽剂中樟脑和苯酚的含量

作  者：
盖静;盖丽;张烨
单  位：
兰州军区联勤部药品仪器检验所;成都军区机关第一门诊部;兰州军区联勤部药品仪器检验所成都军区机关医院
关键词：
高效液相色谱法;复方樟脑搽剂;樟脑;苯酚;含量测定
摘  要：
目的:建立变换波长的方法测定复方樟脑搽剂中樟脑和苯酚的含量。方法:采用高效液相色谱法测定复方樟脑搽剂中樟脑和苯酚的含量,色谱柱Shim-pack VP-ODS(150 mm×4.6 mm,5μm),以甲醇-水(65∶35)为流动相,流速:1.0 mL/min,柱温:25℃,进样量:20μL,检测波长范围:0~5 min为270 nm,5~10 min为288 nm。结果:樟脑和苯酚的线性范围分别为0.051 40~1.028 00 mg/mL(r=0.999 9)与0.052 32~1.046 00 mg/mL(r=0.999 9),平均回收率分别为97.82%(RSD=0.9%)和98.09%(RSD=1.9%)。结论:本法操作简便、结果准确、重现性好,可用于复方樟脑搽剂的质量控制。
译  名：
Determination of camphor and phenol in compound camphor liniment by wavelength conversion HPLC
作  者：
GAI Jing;GAI Li;ZHANG Ye;Institute for Drug and lnstrument Control of Lanzhou Military Region;Hospital of Chengdu Military Region;
关键词：
HPLC;;compound camphor liniment;;camphor;;phenol;;content determination
摘  要：
Objective: A wavelength conversion HPLC method for the determination of camphor and phenol in compound camphor liniment was established. Methods: HPLC method was used for the determination of camphor and phenol in compound camphor liniment with Shim-pack VP-ODS column and methanol-water(65∶35) as mobile phase. The flow rate was 1.0 mL·min~(-1)and column temperature was 25 ℃, sample size: 20 μL. The detection wavelength was set at 270 nm(0~5 min) and 288 nm(5~10min). Results: The linear range were 0.051 40~1.028 00 mg·mL~(-1)(r=0.999 9) and 0.052 32 ~1.046 00 mg/mL(r =0.999 9), for camphor and phenol respectively. The corresponding average recovery rate was 97.82%(RSD=1.5%) and 98.09%(RSD=1.8%).Conclusion: The method is simple, accurate and with good reproducibility, which can be used as quality control for compound camphor liniment.

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