单 位：
南昌航空大学环境与化学工程学院;宁夏大学能源化工重点实验室
摘 要：
试验采用氨基柱(150 mm×4.6 mm,5μm粒径,10nm孔径),流动相A:CH3CN;流动相B:5 mM乙酸铵水溶液;梯度:0～5 min(5%～30%B),50～60 min(30%B);检测波长:270 nm;流速:1.0 mL/min。旨在亲水作用色谱模式下,建立测定红花注射液中腺苷含量的方法。结果表明:腺苷浓度在2.5～20μg/mL范围内,线性关系良好,平均回收率为98.56%,RSD为3.17%(n=9)。该方法简便、快速、准确,结果可靠。
译 名：
Determination of Adenosine in Safflower Injection by Hydrophilic Interaction Liquid Chromatography(HILIC)
作 者：
LI Yuan-yuan1,CHEN Tong2,FANG Jun-zhuo1(1.Key Laboratory of Energy and Chemical Engineering,Ningxia University,Ningxia Yinchuan 750021,China;2.School of Environment and Chemical Engineering,Nanchang Hangkong University,Jiangxi Nanchang 330063,China)
关键词：
Safflower injection;Adenosine;HILIC
摘 要：
The samples were determined by HILIC on a NH2 column(150 mm×4.6 mm,5 μm particle size;10 nm pore size).Conditions: mobile phase A,acetonitrile and mobile phase B,5 mm ammonium acetate aqueous solution.Gradient: 0 to 50 min(5% to 30% B),50 to 60 min(30% B),UV detection: 270 nm and the flow rate was 1.0 ml/min.The aim was to establish HILIC method and subsequently to determine the content of adenosine in Safflower Injection.The result showed that the method gave fine linear correlation from 2.5 μg/ml to 20 μg/ml,the average recovery rate was 98.56% and the RSD 3.17%(n=9).The method is convenient,rapid,accurate and reliable for adenosine determination.
