单 位：
广东省农业科学院蚕业与农产品加工研究所,广东省农产品加工公共实验室;广东省农业科学院蚕业与农产品加工研究所
关键词：
青梅酒;抗氧化作用;Lyoniresinol;DPPH自由基;总抗氧化能力
摘 要：
采用化学模拟体系对青梅酒的总抗氧化能力、清除DPPH自由基能力进行测定;并建立一种用XDB-C18柱(4.6mm×250mm,5μm),以甲醇-水溶液(25:75,V/V),流速1.0ml/min,检测波长202nm,柱温30℃条件下,用HPLC测定Lyoniresinol的方法。结果表明,青梅酒对DPPH自由基清除能力IC50为2.84%,总抗氧化能力(TAC)为(18.785±0.722)mmol/ml(FARP法),青梅酒中Lyoniresinol含量为0.772mg/L。显示青梅酒具有较强的抗氧化能力。
译 名：
Antioxidation and HPLC Determination of Lyoniresinol in Plum Fermented Wine
作 者：
CHEN Zhi-yi,WU Ji-jun,LIU Xue-ming,LI Sheng-feng (Open Access Laboratory of Agricultural Product Processing,Institute of Sericulture and Farm Produce Processing Research,Guangdong Academy of Agricultural Sciences,Guangzhou 510610,China)
关键词：
plum fermented wine;antioxidation;lyoniresinol;DPPH radical;TAC
摘 要：
The total antioxidant capacity (TAC) and DPPH· scavenging activity of plum fermented wine were measured using chemical simulation system. A HPLC method was developed for the determination of content of lyoniresinol in plum fermented wine. The HPLC separation was preformed on a XDB-C18 (4.6 mm × 250 mm,5 μm) at 30 ℃ using a mobile phase composed of a mixture of methanol and water (25:75,V/V) at a flow rate of 1.0 ml/min,and lyoniresinol was detected at 202 nm. The IC50 of scavenging DPPH· radicals was 2.84% and the TAC was measured as being (18.785 ± 0.722) mmol/ml by FARP method. The lyoniresinol content was 0.772 mg/L. In general plum fermented wine has stronger antioxidant activity.
