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当前位置: 首页 > 文章 > 野生二粒小麦低分子量谷蛋白基因序列分析 四川农业大学学报 2008,26 (4) 385-392
Position: Home > Articles > Isolation and Characterization of Two Low Molecular Weight Glutenin Subunit Genes from Triticum dicoccoides Journal of Sichuan Agricultural University 2008,26 (4) 385-392

野生二粒小麦低分子量谷蛋白基因序列分析

作  者：
伍碧华;路洁霏;胡喜贵;李平;颜泽洪;刘登才;郑有良
单  位：
四川农业大学
关键词：
野生二粒小麦;低分子量谷蛋白;基因克隆;序列分析;二级结构
摘  要：
根据低分子量谷蛋白亚基(LMW-GS)基因编码区保守序列设计引物,用PCR方法从蛋白质含量低至13.17%的D81和高达27.20%的D42 2份野生二粒小麦(Triticumdicoccoides)中克隆得到2个LMW-GS基因序列LMW-D81和LMW-D42(GenBank上的序列号分别为FJ461691和FJ461690)。它们具有小麦低分子量谷蛋白基因的典型结构特征,其长度分别为1053bp和1011bp,并分别编码350和336个氨基酸残基的成熟蛋白。LMW-D42和LMW-D81的氨基酸序列估算分子量分别为38kDa和39kDa,说明二者均为C型亚基编码基因。LMW-D42和LMW-D81的N-末端序列都为METSHIP-,表明这2个C型亚基编码基因归属LMW-m型。同源性比对和聚类分析揭示,LMW-D81和LMW-D42均属于Glu-B3位点编码基因。LMW-D42和LMW-D81的核苷酸序列和推导的氨基酸序列一致性分别为93.94%和92.57%。与LMW-D81相比,LMW-D42除发生了22处间断性的碱基替换外,还存在一段42个碱基的缺失。对推导氨基酸序列进行的二级结构预测显示,LMW-D81和LMW-D42的蛋白质二级结构高度一致。其α-螺旋主要位于信号肽和C-末端,少量的α-螺旋和不规则卷曲构成了N-末端。大多数不规则卷曲位于重复区,仅有的一段β-折叠则出现在C-末端。同时,它们的编码区均具有分布一致的8个半胱氨酸残基,且第一和第七个半胱氨酸残基均位于无规则卷曲中。这些结构特点对小麦加工品质改良具有一定意义。
译  名：
Isolation and Characterization of Two Low Molecular Weight Glutenin Subunit Genes from Triticum dicoccoides
作  者：
WU Bi-hua1,2,LU Jie-fei1,2,HU Xi-gui1,2,LI Ping1,2,YAN Ze-hong1,2,LIU Deng-cai1,2,ZHENG You-liang1,2(1.Triticeae Research Institute,Sichuan Agricultural University,Wenjiang 611130,Sichuan,China;2.Key Laboratory of Crop Genetic Resources and Improvement,Ministry of Education,Sichuan Agricultural University,Yaan 625014,Sichuan,China)
关键词：
Triticum dicoccoides;low molecular weight glutenin;gene cloning;sequence analysis;secondary structure
摘  要：
In this paper,PCR for the genomic DNA from Triticum dicoccoides was conducted by using a pair of primers designed according to the conservative domain from the known low molecular weight glutenin subunit genes.The two LMW-GS genes,LMW-D81 and LMW-D42(GenBank accession number FJ461691 and FJ461690),were obtained from the accession D81 with the low protein content at 13.17% and D42 with the high protein content at 27.20%,respectively.LMW-D42 and LMW-D81 possessed the nucleotide sequences with 1011 bp and 1053 bp,and the deduced amino acid sequences with 336 and 350 amino acid residues,respectively.LMW-D42 and LMW-D81 belonged to C type genes owing to their molecular weight about 38 kDa and 39 kDa,respectively,as well as to LMW-m type genes based on the predicted protein sequences of N-terminal domain with METSHIP-.Meanwhile,LMW-D42 and LMW-D81 being allelic Glu-B3 genes were implied by homologous and cluster analyses.The similarities between their sequences were 93.94% in nucleotide and 92.57% in deduced amino acid,respectively.A single deletion for 42 bp occurred in LMW-D42 besides the substitutes at 22 interval sites,compared with LMW-D81.The predicted secondary structures of the amino acid sequences deduced from the two genes were similar.The signal peptide and C-terminal region consisted of many α-helixs.The N-terminal region was formed by a short section of both α-helixs and coils.The repetitive region was mainly occupied by coil.A single β-sheet structure was observed only in C-terminal region.Their amino acid sequences had 8 cysteine residues with similar distribution,and the first and seventh cysteine residues located in the coils.So,these structural characters of LMW-D42 and LMW-D81 might be of importance to quality improvement of bread wheat.

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