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Position: Home > Articles > The Genotype Frequency of Porcine GH Gene in Six Local Breeds (Population) Journal of Henan Agricultural Sciences 2008,37 (12) 127-130

6个地方猪品种(类群)GH基因的基因型分布研究

作  者:
张依裕;胡尚;陈国宏;张海波
单  位:
贵阳市花溪区农业局;贵州大学;扬州大学
关键词:
猪;生长激素基因;PCR-RFLP;限制性片断长度多态性
摘  要:
采用PCR-RFLP技术对6个地方品种(类群)GH基因-119bp至+486bp区域进行扩增,分别用内切酶ApaⅠ和Hin6Ⅰ检测其多态性。结果表明:ApaⅠ酶切产生2个等位基因A和B,3种基因型AA,AB和BB,AA为优势基因型,A为优势等位基因;Hin6Ⅰ酶切产生3个等位基因C2,C3和C4,4种基因型C2C3,C2C4,C3C4和C4C4,C4C4为优势基因型,C4为优势等位基因。ApaⅠ和Hin6Ⅰ酶切基因型在猪群体间的分布不均匀,且差异都达到极显著水平(P<0.01)。
译  名:
The Genotype Frequency of Porcine GH Gene in Six Local Breeds (Population)
作  者:
ZHANG Yi-yu1,HU Shang2,CHEN Guo-hong3,ZHANG Hai-bo3 (1.College of Animal Science of Guizhou University,Guiyang 550025,China;2.Agricultural Bureau of Huaxi,Gui yang 550025,China;3.College of Animal Science and Technology of Yangzhou University,Yangzhou 225009,China)
关键词:
Pig;Growth hormone gene;PCR-RFLP;Restriction fragment length polymorphism
摘  要:
In this study,the polymorphisms from-119bp to +486bp of growth hormone gene in six local pig breeds(populations) were investigated by PCR-RFLP.2 alleles(A and B) and 3 genotypes(AA,AB and BB) were produced when the PCR products were digested by ApaⅠ,of which A and AA were predominant.When digested by Hin6Ⅰ,3 alleles(C2,C3 and C4) and 4 genotypes(C2C3,C2C4,C3C4 and C4C4) were produced,among which C4 and C4C4 were predominant allele or genotype.The results showed that the genotype distribution in six local breeds(populations) are significant different(P<0.01).

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