关键词：
山月桂;茎段;组织培养;快速繁殖;珍珠岩
摘 要：
生根困难一直是影响山月桂应用的主要问题,本研究以山月桂‘奥运圣火’带芽茎段为外植体,研究基本培养基、植物生长调节剂,以及珍珠岩对其腋芽萌发、组培苗的增殖、伸长和生根的影响。结果表明:最适合茎段腋芽萌发的培养基为WPM+1.0 mg/L 2-ip,萌发率可达72.5%;组培苗增殖的最佳培养基为WPM+1.0 mg/L 2-ip+0.05 mg/L NAA,增殖系数可达5.42,平均苗高达3.42 cm;最适合的生根培养基为:1/2 WPM+1.0 mg/L IBA+0.1 mg/L NAA+珍珠岩,生根率80.03%。将已经生根的组培苗炼苗后移栽到泥炭土和珍珠岩(1∶1,体积比)的混合基质中,成活率在60%左右。
译 名：
Tissue Culture and Highly Efficient Rooting of Kalmia latifolia ‘Olympic Fire'
作 者：
XING Wen;CAI Mengying;ZENG Wen;CAI Yixia;JIN Xiaoling;College of Landscape Horticulture,Central South University of Forestry and Technology;
关键词：
Kalmia latifolia;;nodal segments;;tissue culture;;rapid proliferation;;erlite
摘 要：
Difficulty in root generation hinders the application of Kalmia latifolia. By in vitro culture of nodal segments of K. latifolia ‘Olympic Fire',the effects of basal medium,plant growth regulators and perlite on rooting for rapid propagation were investigated. The results indicated that WPM basal medium supplemented with 2-ip 1. 0 mg / L significantly improved bud break and growth directly from nodal segment explants,and the sprouting rate was about 72. 5%. The optimal medium for shoot multiplication was WPM + 1. 0 mg / L 2-ip + 0. 05 mg / L NAA,on which the multiplication coefficient reached 5. 42 and the average height of shoots were 3. 42 cm. Rooting of shoots was optimized on halfstrength WPM medium with 1. 0 mg / L IBA + 0. 1 mg / L NAA combined with perlite,on which the rooting rate was 80. 03%. After acclimatization,about 60% of rooting plantlets could survive when transplanted into the matrix composed of peat soil and perlite( 1∶ 1,volume ratio).
