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当前位置: 首页 > 文章 > 牦牛源牛冠状病毒部分基因的扩增、序列分析及病毒分离鉴定 畜牧兽医学报 2019 (2) 343-353
Position: Home > Articles > Amplification,Sequence Analysis of Bovine Coronavirus Genes and Isolation of the Viruses from Yak Chinese Journal of Animal and Veterinary Sciences 2019 (2) 343-353

牦牛源牛冠状病毒部分基因的扩增、序列分析及病毒分离鉴定

作  者：
何琪富;汤承;郭紫晶;谭烁;张斌;岳华
单  位：
西南民族大学生命科学与技术学院;青藏高原动物遗传资源保护与利用教育部重点实验室
关键词：
牛冠状病毒;流行病学;分子特征;分离;S基因重组;牦牛
摘  要：
本试验的目的是对青藏高原地区牦牛进行牛冠状病毒(bovine coronavirus,BCoV)的分子流行病学调查,并分离牦牛源BCoV。采用RT-PCR方法检测西藏、青海、四川、云南的犊牦牛腹泻粪便中BCoV,并扩增其S、HE及N基因片段;选取阳性样本进行BCoV分离。结果显示:从336份犊牦牛腹泻粪便中检出232份BCoV阳性,检出率为69.05%。序列分析和系统发育分析显示,本试验克隆的32个BCoV阳性样本中的S1亚基序列、HE基因片段和N基因片段均有独特的遗传进化趋势;首次成功分离到1株牦牛源BCoV,蚀斑纯化后病毒TCID50为10-7.17·0.1mL-1,鉴定结果显示该毒株S基因发生了重组事件。本试验结果表明青藏高原牦牛源BCoV感染率很高,且有独特的遗传进化趋势。
译  名：
Amplification,Sequence Analysis of Bovine Coronavirus Genes and Isolation of the Viruses from Yak
作  者：
HE Qifu;TANG Cheng;GUO Zijing;TAN Shuo;ZHANG Bin;YUE Hua;College of Life Science and Technology,Southwest University for Nationalities;Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization;
单  位：
HE Qifu%TANG Cheng%GUO Zijing%TAN Shuo%ZHANG Bin%YUE Hua%College of Life Science and Technology,Southwest University for Nationalities%Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization
关键词：
bovine coronavirus;;prevalence;;molecular characterization;;isolation;;S gene recombination;;yak
摘  要：
The aim of this study was to investigate molecular prevalence of bovine coronavirus(BCoV)in yak in Qinghai-Tibetan Plateau and to isolate BCoV from clinical samples.RT-PCR assay was used to detect BCoV from 336 diarrhea feces samples of yak from Tibet,Qinghai,Sichuan and Yunnan,and the S,HE,and N gene fragments were further amplified,moreover,positive samples were used to isolate BCoV strain.Results were as follows:Approximate69.05%(232/336)diarrheic samples were detected BCoV positive by RT-PCR.Sequence and phylogenetic analysis showed that BCoV S1 subunit sequence,HE gene fragment and N gene fragment from 32 clinical samples in this study had unique evolutionary features.Notably,a BCoV strain of yak was successfully isolated firstly,the TCID50 of this strain was 10-7.17·0.1 mL-1,and a recombinant event was predicted in Sgene of this strain.These results indicate that BCoV widely circulates in yak in the Qinghai-Tibetan Plateau of China and exhibit unique evolution.

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