Position: Home > Articles > EFFECT OF 8 METAL IONS ON PATHOGENIC FACTORS OF SOFT ROT ERWINIA
Acta Phytopathologica Sinica
1995
(1)
79-84
Ca~(2+)、Mn~(2+)等8种金属离子对软腐欧文氏菌一些致病因子的影响
作 者：
张学君;王金生;方中达
单 位：
南京农业大学植保系
关键词：
软腐欧文氏菌;金属离子;胞外酶;酶活性;致病因子
摘 要：
在离体试验中,不同金属离子对马铃薯软腐欧文氏菌(Erwinia carotovora subsp.carotovora)菌株StEcc-12生长,产生胞外酶和胞外酶活性具有不同的影响。在含果胶酸钠的培养液中,Ca~(2+)促进生长,并使培养液中的果胶裂解酶(PL)、果胶水解酶(PG)和蛋白酶3种胞外酶分别提高1.4～23.4倍,0.3～2.9倍和0.7～6.5倍。Mn~(2+)对StEcc-12生长及产生3种胞外酶的影响与Ca~(2+)相似。Ni~(2+)显著抑制细菌的生长,但在含Ni~(2+)培养液中3种胞外酶的单位活性比对照高1.7倍以上。在酶粗提液中分别加入上述3种离子对PL酶的活性均有抑制作用。Zn~(2+)对细菌生长没有显著影响,高浓度Al~(3+)抑制细菌生长,这2种离子均能促进3种胞外酶的产生。随着Fe~(2+)和K~+浓度的增高,细菌生长减少,两种果胶酶产量降低,蛋白酶产量增加,粗提液中PL酶活性下降。Mg~(2+)对细菌生长和胞外PG产生没有明显影响,提高Mg~(2+)浓度有利于PL的产生。酶粗提液中加入Mg~(2+)后PL活性增高。所有以上离子都能使聚半乳糖醛酸发生不同程度的凝聚,但只有Ca~(2+)和Mg~(2+)的有效凝聚浓度与薯块的实际浓度接近。
译 名：
EFFECT OF 8 METAL IONS ON PATHOGENIC FACTORS OF SOFT ROT ERWINIA
作 者：
Zhang Xuejun Wang Jinsheng Fang Zhongda (Dept. of Plant Protection, Nanjing Agric. Univ. nanjing, 210095)
关键词：
Soft rot Erwinia Pathogenic factors Metel ions Extracellular enzyme Enzyme activity
摘 要：
Different metal ions had various effect on in vitro growth production of extracellular enzymes and enzyme activities of strain StEcc-12 of potato soft rot ( Erwinia carotovora subsp. carotovora ). In liquid media containing polygalacturonate, Ca2+ increased growth of StEcc-12 and raised its production of extracellular pectic lyase (PL), polygalacturonase (PG) and protease up to 1. 4~23. 5, 0. 3~2. 9 and 0. 7~6. 5 times, respectively. Mn2+ had the same effect as Ca2+ on bacterial growth and production of 3 extracellular enzymes. Ni2+ inhibited significantly the growth of pathogen, but the unit activities of 3 extracellular enzymes in suspension containing Ni2+ is 1. 7 times higher than check. Addition of above 3 ions alone into a crude extraction inhibited PL activities. Zn2+ han no effect on bacterial growth, and higher content of A13+ inhibited, but these 2 ions increased production of extracellular enzymes. As Fe2+ and K+ increased, bacterial growth and PL activities in extract solution decreased, synthesis of PL and PG reduced, but protease production raised. Mg2+ had no significant effect on in vitro growth and extracellular PG. Increased Mg2+promoted PL synthesis and its activities. All 8 ions could agglutate poly-galacturonic sodium, but only Ca2+ and Mg2+ effectively binding it at a content similar to those in potato tubers (102. 2ppm and 179. 2ppm, respectively).
相似文章
-
软腐欧文氏菌致病性的研究进展 [贾云鹤, 张俊华, 崔崇士] 东北农业大学学报 2007,38 (1) 113-118
