Isolation and identification of a strain of Cyprinid Herpesvirus 2 in Sheyang

LI Mao;XIAO Dan;LIU Tian-qiang;LIU Yan-peng;HUANG Guan-jun;WANG Kai-yu;LUO Yuan-hui;The Animal Health Institute,Tongwei Co. Ltd.;Fisheries Department,Sichuan Agricultural University;

Cy HV-2;;Carassius auratus gibelio;;Isolation;;identification

In order to verify that the isolated virus was Cy HV-2 virus,the test of the gill hemorrhage disease of Carassius auratus gibelio lesions were isolated and identified by recurrent infection test,isolation and identification,electron microscope observation and PCR for identification of herpes simplex Ⅱtype virus. The results showed: Health C. auratus gibelio injection of tissue filtrates of 5 d after the beginning of the disease,and symptoms of the primary disease,the mortality rate was 80%,the control group had no death. The prevalence of fish viscera tissues by ultrathin sections within the organization,found that a large number of virus particles observed by electronic microscope,mature virus particle diameters of 170~ 200 nm,and herpes simplex Ⅱtype virus particle match. Detection of the filtrate by herpes simplex Ⅱtype virus specific polymerase chain reaction( PCR),and detected the positive fragment of 436 bp,at the same time the filtrate inoculation fat carp cell disease like aseptic processing( FHM),after 1 d culture appears obvious cytopathic( CPE),cell monolayer mesh shrinkage,necrosis cell aggregation. The collection of cell culture fluid,detection of virus specific polymerase chain reaction( PCR),and detected the positive fragment of 436 bp. Conclusion: All of the evidences indicated that the isolated virus stain was Cy HV-2.